Ruby J C, Martin C L, Muller H K
Int Arch Allergy Appl Immunol. 1984;73(3):198-204. doi: 10.1159/000233467.
Using flow cytofluorimetry histamine binding was demonstrated by 90-100% of human peripheral blood lymphocytes. Cells were labelled with fluorescein isothiocyanate-human albumin-histamine (FITC-HA-His) and analysed using a Fluorescence Activated Cell Sorter (FACS-II). A parallel control FITC-HA conjugate and blocking with both free histamine and histamine bound to albumin were used to establish specificity. The binding of histamine could be blocked with both H1 and H2 type histamine antagonists. Nylon wool separated T and B lymphocyte subpopulations showed the same high proportions of histamine binding cells.
采用流式细胞荧光测定法，90%-100%的人外周血淋巴细胞可显示组胺结合。细胞用异硫氰酸荧光素-人白蛋白-组胺（FITC-HA-His）标记，并使用荧光激活细胞分选仪（FACS-II）进行分析。采用平行对照FITC-HA共轭物以及游离组胺和与白蛋白结合的组胺进行封闭，以确定其特异性。组胺的结合可被H1型和H2型组胺拮抗剂阻断。经尼龙毛分离的T和B淋巴细胞亚群显示出相同比例的高组胺结合细胞。
