Imshenetskiĭ A A, Lysenko S V, Kozlova T M, Novichkova A T
Mikrobiologiia. 1983 Nov-Dec;52(6):902-8.
The object of this work was to study cytological changes caused by periodic freezing-thawing in the conidia of the fungi Aspergillus niger and Penicillium chrysogenum isolated from the mesosphere as well as in the conidia of the same species taken from the collection of microorganisms. The conidia from the mesosphere were found to be highly resistant to the treatment. As was shown by electron microscopy, the outer "backbone" layer of the spore envelope broke down and the membranes of the plasmalemma stratified after ten cycles in A. niger and P. chrysogenum. The percentage of conidia with the damaged ultrastructural organization was higher in mutants of these cultures.
这项工作的目的是研究从中间层分离出的黑曲霉和产黄青霉的分生孢子以及从微生物保藏中心获取的相同物种的分生孢子,在周期性冻融作用下所引起的细胞学变化。结果发现,来自中间层的分生孢子对这种处理具有高度抗性。电子显微镜观察显示,在黑曲霉和产黄青霉中,经过十次循环后,孢子包膜的外层“骨架”层破裂,质膜分层。在这些培养物的突变体中,超微结构组织受损的分生孢子百分比更高。