Garzo V G, Dorrington J H
Am J Obstet Gynecol. 1984 Mar 1;148(5):657-62. doi: 10.1016/0002-9378(84)90769-5.
Granulosa cells were isolated from follicles that ranged in size from 0.4 to 2.0 cm in diameter, obtained from patients during the follicular phase of the menstrual cycle. Aromatase activity, assessed by the release of tritiated water from [1 beta-3H]testosterone, was undetectable in follicles with a diameter of less than 1.0 cm; thereafter, there was a direct correlation between aromatase activity and follicular diameter. When cultured in the presence of 200 ng of National Institutes of Health follicle-stimulating hormone-15 per milliliter, aromatase activity was stimulated in cells isolated from all sizes of follicles from 0.4 to 1.5 cm. Insulin (500 ng/ml) further augmented follicle-stimulating hormone (FSH)-induced aromatase activity, as evidenced both by an increase in 17 beta-estradiol production and by the release of tritiated water from [1 beta-3H]testosterone by granulosa cells. This study further delineates the role of FSH in estrogen production during human follicular development and suggests that insulin or insulin-like growth factors may play a role in modifying the FSH-dependent cellular differentiation of human granulosa cells.
颗粒细胞从直径为0.4至2.0厘米的卵泡中分离出来，这些卵泡取自月经周期卵泡期的患者。通过[1β-3H]睾酮释放出的氚化水来评估芳香化酶活性，在直径小于1.0厘米的卵泡中未检测到该活性；此后，芳香化酶活性与卵泡直径之间存在直接相关性。当在每毫升含有200纳克美国国立卫生研究院促卵泡激素-15的条件下培养时，从直径0.4至1.5厘米的各种大小卵泡中分离出的细胞的芳香化酶活性均受到刺激。胰岛素（500纳克/毫升）进一步增强了促卵泡激素（FSH）诱导的芳香化酶活性，这通过颗粒细胞产生的17β-雌二醇增加以及[1β-3H]睾酮释放出氚化水得以证明。这项研究进一步阐明了FSH在人类卵泡发育过程中雌激素产生中的作用，并表明胰岛素或胰岛素样生长因子可能在调节人类颗粒细胞依赖FSH的细胞分化中发挥作用。
