Boutin J A, Siest G, Batt A M, Solheim E, Scheline R R
Anal Biochem. 1983 Nov;135(1):201-7. doi: 10.1016/0003-2697(83)90752-2.
A method for the assay of uridine diphosphate (UDP)-glucuronosyltransferase activities toward some phenolic compounds and monoterpenoid alcohols is described. The method is based on the disappearance of the free substrate after incubation with microsomes and UDP-glucuronate. This disappearance is recorded using a gas chromatographic process. This method has been used, for example, to characterize the glucuronidation process of eugenol (4-allyl-2-methoxyphenol). The method could be extended to other substrates. Analytical conditions are given for some of them, especially monoterpenoid alcohols since the studies of their conjugations are a growing field of interest in evaluation of heterogeneity of UDP-glucuronosyltransferase. The method could also be used with other biological materials including cell suspension and crude liver biopsies.
本文描述了一种用于测定尿苷二磷酸（UDP）-葡糖醛酸基转移酶对某些酚类化合物和单萜醇活性的方法。该方法基于与微粒体和UDP-葡糖醛酸孵育后游离底物的消失。这种消失通过气相色谱法记录。例如，该方法已用于表征丁香酚（4-烯丙基-2-甲氧基苯酚）的葡糖醛酸化过程。该方法可扩展到其他底物。给出了其中一些底物的分析条件，特别是单萜醇，因为它们的结合研究是UDP-葡糖醛酸基转移酶异质性评估中一个日益受关注的领域。该方法也可用于其他生物材料，包括细胞悬液和肝活检粗提物。
