Law S W, Gray G, Brewer H B, Sakaguchi A Y, Naylor S L
Biochem Biophys Res Commun. 1984 Feb 14;118(3):934-42. doi: 10.1016/0006-291x(84)91485-2.
Apolipoprotein (apo) A-I is a major protein of high density lipoproteins (HDL). The gene for apoA-I has been localized to the p11 leads to q13 region of chromosome 11 by filter hybridization analysis of mouse-human hybrid cell cDNAs containing chromosome 11 translocations utilizing a cloned human apoA-I cDNA probe. The known linkage of apoA-I and apoC-III also permitted the simultaneous assignment of the apoC-III gene to the same region on chromosome 11. Comparison with previously established gene linkages on the mouse and human genome suggests that apoA-I + apoC-III may be linked to the esterase A4 and uroporphyrinogen synthase genes which are present on the long arm of human chromosome 11. The localization of the apoA-I + apoC-III genes in the p11----q13 region of chromosome 11 represents a definitive chromosomal assignment of a human apolipoprotein gene, and will now enable more detailed analysis of the geneomic organization and linkages of the apolipoprotein genes.
载脂蛋白（apo）A-I是高密度脂蛋白（HDL）的主要蛋白质。通过利用克隆的人apoA-I cDNA探针，对含有11号染色体易位的小鼠-人杂交细胞cDNA进行滤膜杂交分析，已将apoA-I基因定位到11号染色体的p11至q13区域。已知的apoA-I和apoC-III的连锁关系也使得apoC-III基因能够同时定位到11号染色体的同一区域。与小鼠和人类基因组中先前确定的基因连锁关系进行比较表明，apoA-I + apoC-III可能与存在于人类11号染色体长臂上的酯酶A4和尿卟啉原合酶基因连锁。apoA-I + apoC-III基因在11号染色体p11----q13区域的定位代表了人类载脂蛋白基因明确的染色体定位，现在将能够对载脂蛋白基因的基因组组织和连锁关系进行更详细的分析。
