Perrard M H, Saez J M, Dazord A
FEBS Lett. 1984 Mar 12;168(1):49-53. doi: 10.1016/0014-5793(84)80204-5.
We studied the effect of FHS on nuclear protein synthesis by a primary culture of immature porcine Sertoli cells. The cells were cultured in a chemically defined medium and treated with FSH (50 ng/ml) for various periods (4, 8, 25 and 64 h). About 65 spots (pHi less than 8) were identified by two-dimensional polyacrylamide gel electrophoresis of the radiolabelled nuclear proteins. After 25 h of FSH treatment, we observed an increase of 5 proteins, a slight decrease of two and a disappearance of one. For the short periods of FSH treatment (4 and 8 h), no effect of FHS on nuclear protein synthesis was observed. After 64 h of FSH treatment (4 and 8 h), no effect of FHS on nuclear protein synthesis was observed. After 64 h of FSH treatment, the synthesis of all nuclear proteins appears to be decreased.
我们通过原代培养未成熟猪支持细胞研究了促卵泡激素（FHS）对核蛋白合成的影响。细胞在化学成分明确的培养基中培养，并用促卵泡激素（FSH，50纳克/毫升）处理不同时间段（4、8、25和64小时）。通过对放射性标记的核蛋白进行二维聚丙烯酰胺凝胶电泳，鉴定出约65个斑点（pH值小于8）。促卵泡激素处理25小时后，我们观察到5种蛋白质增加，2种略有减少，1种消失。在促卵泡激素处理的短时间内（4和8小时），未观察到促卵泡激素对核蛋白合成的影响。促卵泡激素处理64小时后，未观察到促卵泡激素对核蛋白合成的影响。促卵泡激素处理64小时后，所有核蛋白的合成似乎都减少了。
