Burgos-Briceño L A, Smith C G, Coy D H, Schally A V, Asch R H
Acta Eur Fertil. 1983 Jul-Aug;14(4):255-60.
The pulsatility of luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion was evaluated in chronically ovariectomized rhesus monkeys before and after the administration of [N-Ac-D-Trp1, 3, D-p-Cl-Phe2, D-Phe6, D-Ala10]-LH-RH) (IA-LHRH). Animals were divided into three groups (n = 4, each group) receiving, s.c.: 1) vehicle only (controls); 2) 0.5 mg IA-LH-RH; and 3) 2 mg IA-LH-RH. Twelve hours following drug administration, animals were chair-restrained and blood samples drawn every 10 minutes over a period of 4 hours via indwelling femoral vein catheters. LH and FSH levels were measured by RIA to determine the frequency, amplitude and increment of the pulses. Twelve hours after the administration of 0.5 mg or 2.0 mg of IA-LH-RH, levels of LH were reduced by 30 per cent and 68 per cent (P less than 0.001) and those of FSH by 34 per cent and 53 per cent (P less than 0.001 and P less than 0.002), respectively. Doses of 0.5 mg IA-LH-RH decreased the amplitude (20 per cent; P less than 0.001), increment (17 ng/ml; P less than 0.001) and frequency (2 per 4 h; P less than 0.001) of LH pulses. Administration of 2 mg IA-LH-RH produced a fall of increment (8 ng/ml; P less than 0.001), frequency (1 per 4 h; P less than 0.001) and pulse amplitude (26 per cent; P less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
在长期卵巢切除的恒河猴中，在给予[N-乙酰-D-色氨酸1,3，D-对氯苯丙氨酸2，D-苯丙氨酸6，D-丙氨酸10]-促黄体激素释放激素（IA-LHRH）之前和之后，评估促黄体生成素（LH）和促卵泡激素（FSH）分泌的脉冲性。将动物分为三组（每组n = 4），皮下注射：1）仅注射赋形剂（对照组）；2）0.5mg IA-LH-RH；3）2mg IA-LH-RH。给药12小时后，将动物用椅子固定，并通过留置的股静脉导管在4小时内每隔10分钟采集一次血样。通过放射免疫分析法测量LH和FSH水平，以确定脉冲的频率、幅度和增量。给予0.5mg或2.0mg IA-LH-RH 12小时后，LH水平分别降低了30%和68%（P<0.001），FSH水平分别降低了34%和53%（P<0.001和P<0.002）。0.5mg IA-LH-RH剂量降低了LH脉冲的幅度（20%；P<0.001）、增量（17ng/ml；P<0.001）和频率（每4小时2次；P<0.001）。给予2mg IA-LH-RH导致增量下降（8ng/ml；P<0.001）、频率下降（每小时1次；P<0.001）和脉冲幅度下降（26%；P<0.001）。（摘要截短于250字）
