Polypeptide microsequence analysis with the commercially available gas-phase sequencer.

An evaluation of the commercially available gas-phase sequencer with regard to its sensitivity, ease of operation, and reliability is presented. Techniques for the preparation of ultra-pure reagents and solvents for this new technology are reviewed, as is a highly sensitive reverse-phase liquid chromatography program for the identification and quantitation of phenylthiohydantoin amino acid residues from the new sequenator.