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使用市售气相测序仪进行多肽微序列分析。

Polypeptide microsequence analysis with the commercially available gas-phase sequencer.

作者信息

Esch F S

出版信息

Anal Biochem. 1984 Jan;136(1):39-47. doi: 10.1016/0003-2697(84)90305-1.

DOI:10.1016/0003-2697(84)90305-1
PMID:6424503
Abstract

An evaluation of the commercially available gas-phase sequencer with regard to its sensitivity, ease of operation, and reliability is presented. Techniques for the preparation of ultra-pure reagents and solvents for this new technology are reviewed, as is a highly sensitive reverse-phase liquid chromatography program for the identification and quantitation of phenylthiohydantoin amino acid residues from the new sequenator.

摘要

本文对市售气相测序仪的灵敏度、操作简便性和可靠性进行了评估。综述了用于这项新技术的超纯试剂和溶剂的制备技术，以及一种用于从新型测序仪中鉴定和定量苯硫基乙内酰脲氨基酸残基的高灵敏度反相液相色谱程序。

相似文献

1
Polypeptide microsequence analysis with the commercially available gas-phase sequencer.使用市售气相测序仪进行多肽微序列分析。
Anal Biochem. 1984 Jan;136(1):39-47. doi: 10.1016/0003-2697(84)90305-1.
2
Utility of the gas-phase sequencer for both liquid- and solid-phase degradation of proteins and peptides at low picomole levels.气相测序仪在低皮摩尔水平下用于蛋白质和肽的液相及固相降解的效用。
Anal Biochem. 1984 Aug 1;140(2):553-66. doi: 10.1016/0003-2697(84)90207-0.
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Solid-phase sequencing on the gas-phase sequencer.气相测序仪上的固相测序
Anal Biochem. 1986 May 1;154(2):542-51. doi: 10.1016/0003-2697(86)90027-8.
4
Conversion of the beckman liquid phase sequencer to a gas-liquid phase sequencer.
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[Sequential Edman degredation].[连续的埃德曼降解法]
Biochimie. 1977;59(7):557-76. doi: 10.1016/s0300-9084(77)80166-1.
6
Microsequence analysis of peptides and proteins. V. Design and performance of a novel gas-liquid-solid phase instrument.
Anal Biochem. 1985 Jun;147(2):315-30. doi: 10.1016/0003-2697(85)90278-7.
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High sensitivity automated sequence determination of polypeptides.多肽的高灵敏度自动序列测定
J Biol Chem. 1975 May 25;250(10):3629-36.
8
Protected edman degradation: a new approach to microsequence analysis of proteins.保护型埃德曼降解法：蛋白质微序列分析的新方法。
FEBS Lett. 1974 Apr 15;41(1):62-4. doi: 10.1016/0014-5793(74)80954-3.
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High-pressure liquid chromatographic analysis of amino acid phenylthiohydantoins: comparison with other techniques.氨基酸苯硫代乙内酰脲的高压液相色谱分析：与其他技术的比较。
Anal Biochem. 1976 Aug;74(2):298-319. doi: 10.1016/0003-2697(76)90211-6.
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A simple and inexpensive high pressure liquid chromatographic system as an adjunct to gas chromatography in identification of amino acid phenylthiohydantoins.一种简单且廉价的高压液相色谱系统，作为气相色谱的辅助手段用于氨基酸苯硫代乙内酰脲的鉴定。
Arch Biochem Biophys. 1976 Mar;173(1):320-5. doi: 10.1016/0003-9861(76)90265-4.

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