Bonissol C, Traincard F, Stoïljkovic B, Hosli P
Ann Microbiol (Paris). 1984 Jan-Feb;135A(1):63-72. doi: 10.1016/s0769-2609(84)80060-5.
The importance of cell culture contamination by mycoplasmas is well recognized, but the means used to detect such contamination still need improvement. Most mycoplasmas possess an enzyme, adenosine phosphorylase, which is not found in cell lines. We used the ultramicromethod of Uitendaal et al. to detect the presence of mycoplasmas in sera and in tissue culture medium. The absence of adenosine phosphorylase activity seems to be the best guarantee that a serum is not contaminated by mycoplasmas. This test is also most efficient for the detection of mycoplasmas in tissue or cell cultures in vitro.
支原体对细胞培养造成污染的重要性已得到广泛认可，但用于检测此类污染的方法仍需改进。大多数支原体都有一种细胞系中不存在的酶，即腺苷磷酸化酶。我们采用了于滕达尔等人的超微量方法来检测血清和组织培养基中支原体的存在。腺苷磷酸化酶活性的缺失似乎是血清未被支原体污染的最佳保证。该检测方法对于体外检测组织或细胞培养物中的支原体也最为有效。
