Rudkin B B, Mamont P S, Seiler N
Biochem J. 1984 Feb 1;217(3):731-41. doi: 10.1042/bj2170731.
Hepatoma tissue-culture (HTC) cells were exposed to DL-alpha-difluoromethylornithine (DFMeOrn), a specific irreversible inhibitor of ornithine decarboxylase. Concomitantly with the decrease in spermidine, a decrease in the amount of ribosomes in polyribosomes was observed. Spermine concentrations remained essentially comparable with those in cells not exposed to this inhibitor. Exposure of putrescine- and spermidine-depleted HTC cells to spermidine or spermine rapidly reversed the effect of DFMeOrn on polyribosome profiles, whereas addition of putrescine to the cell culture medium had an effect only after its transformation into spermidine and spermine. The results show that the perturbation of polyribosome formation in DFMeOrn-treated HTC cells is due to spermidine deficiency and that a normal polyamine complement is required for optimal protein-synthetic activity in these cells. The results also indicate that protein synthesis is perturbed before DNA synthesis during depletion of putrescine and spermidine in HTC cells.
将肝癌组织培养（HTC）细胞暴露于鸟氨酸脱羧酶的特异性不可逆抑制剂DL-α-二氟甲基鸟氨酸（DFMeOrn）。伴随着亚精胺含量的减少，观察到多核糖体中核糖体数量的减少。精胺浓度与未暴露于该抑制剂的细胞中的浓度基本相当。将腐胺和亚精胺耗尽的HTC细胞暴露于亚精胺或精胺中，可迅速逆转DFMeOrn对多核糖体图谱的影响，而向细胞培养基中添加腐胺只有在其转化为亚精胺和精胺后才有作用。结果表明，DFMeOrn处理的HTC细胞中多核糖体形成的扰动是由于亚精胺缺乏，并且正常的多胺补充对于这些细胞中的最佳蛋白质合成活性是必需的。结果还表明，在HTC细胞中腐胺和亚精胺耗尽期间，蛋白质合成在DNA合成之前就受到了扰动。
