Liskay R M, Kornfeld B, Fullerton P, Evans R
J Cell Physiol. 1980 Sep;104(3):461-7. doi: 10.1002/jcp.1041040318.
V79-8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 mu/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79-8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficient to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measureable G1 interval.
V79 - 8细胞在正常生长条件下缺乏可测量的G1期。我们发现，使用低水平的环己酰亚胺（0.05μg/ml）部分抑制蛋白质合成可在这些细胞中诱导出可测量的G1期，而对S期、G2期或M期没有任何显著影响。鉴于这些发现，对从V79 - 8细胞系中选出的每个表达G1期的隐性突变体进行了蛋白质合成以及降解/损失速率的分析。四个突变体中的三个显示出蛋白质合成速率降低，这足以解释它们的G1期长度。然而，第四个突变体的蛋白质合成以及降解/损失速率与亲本相同。这些结果不仅表明蛋白质合成速率降低可导致G1期的表达，而且表明除了那些简单影响整体蛋白质合成的改变（突变）之外，其他改变也可导致可测量的G1期的表达。
