多花黑麦草胚乳细胞中阿拉伯半乳聚糖蛋白的生物合成。半乳糖基转移酶的亚细胞分布。
Biosynthesis of arabinogalactan-protein in Lolium multiflorum (Italian ryegrass) endosperm cells. Subcellular distribution of galactosyltransferases.
作者信息
Schibeci A, Pnjak A, Fincher G B
出版信息
Biochem J. 1984 Mar 1;218(2):633-6. doi: 10.1042/bj2180633.
Abstract
Intracellular membranes from protoplasts of Italian-ryegrass (Lolium multiflorum) endosperm cells have been fractionated on sucrose density gradients and identified on the basis of putative-marker-enzyme assays. Galactosyltransferases capable of incorporating galactose from UDP galactose into 66% ethanol-soluble products are associated with all membrane fractions. Affinity chromatography of the ethanol-insoluble products on (murine myeloma protein J539)-Sepharose reveals that the enzymes responsible for the synthesis of polymers containing (1----6)-beta-D-galactose residues are associated exclusively with subcellular fractions enriched in Golgi-derived membranes. This suggests that the Golgi apparatus plays an important part in the synthesis of the carbohydrate component of the ryegrass arabinogalactan-protein.
摘要
对意大利黑麦草（多花黑麦草）胚乳细胞原生质体的细胞内膜进行了蔗糖密度梯度分级分离，并根据假定的标记酶测定法进行了鉴定。能够将来自UDP-半乳糖的半乳糖掺入66%乙醇可溶产物中的半乳糖基转移酶与所有膜级分相关。乙醇不溶产物在（鼠骨髓瘤蛋白J539）-琼脂糖凝胶上的亲和层析表明，负责合成含有（1→6）-β-D-半乳糖残基聚合物的酶仅与富含高尔基体衍生膜的亚细胞级分相关。这表明高尔基体在黑麦草阿拉伯半乳聚糖蛋白的碳水化合物成分合成中起重要作用。
相似文献
1
Biosynthesis of arabinogalactan-protein in Lolium multiflorum (Italian ryegrass) endosperm cells. Subcellular distribution of galactosyltransferases.多花黑麦草胚乳细胞中阿拉伯半乳聚糖蛋白的生物合成。半乳糖基转移酶的亚细胞分布。
Biochem J. 1984 Mar 1;218(2):633-6. doi: 10.1042/bj2180633.
2
Localization of beta-glucan synthases on the membranes of cultured Lolium multiflorum (ryegrass) endosperm cells.β-葡聚糖合酶在多花黑麦草（黑麦草）胚乳细胞培养膜上的定位。
Biochem J. 1983 Mar 1;209(3):627-33. doi: 10.1042/bj2090627.
3
Isolation of plasma membrane from protoplasts of Lolium multiflorum (ryegrass) endosperm cells.从多花黑麦草（黑麦草）胚乳细胞原生质体中分离质膜
Biochem J. 1982 Sep 1;205(3):511-9. doi: 10.1042/bj2050511.
4
Biosynthesis of Arabinogalactan-Protein in Lolium multiflorum (Ryegrass) Endosperm Cells : III. Subcellular Distribution of Prolyl Hydroxylase.多花黑麦草（黑麦草）胚乳细胞中阿拉伯半乳聚糖蛋白的生物合成：III. 脯氨酰羟化酶的亚细胞分布
Plant Physiol. 1983 Jul;72(3):754-8. doi: 10.1104/pp.72.3.754.
5
Characterization of the hydroxyproline-rich protein core of an arabinogalactan-protein secreted from suspension-cultured Lolium multiflorum (Italian ryegrass) endosperm cells.对多花黑麦草（意大利黑麦草）胚乳细胞悬浮培养物分泌的阿拉伯半乳聚糖蛋白富含羟脯氨酸蛋白核心的表征。
Biochem J. 1989 Dec 15;264(3):857-62. doi: 10.1042/bj2640857.
6
Galactosyltransferases from Arabidopsis thaliana in the biosynthesis of type II arabinogalactan: molecular interaction enhances enzyme activity.拟南芥中的半乳糖基转移酶在II型阿拉伯半乳聚糖生物合成中的作用：分子相互作用增强酶活性
BMC Plant Biol. 2014 Apr 3;14:90. doi: 10.1186/1471-2229-14-90.
7
Two enzymes involved in the synthesis of O-linked oligosaccharides are localized on membranes of different densities in mouse lymphoma BW5147 cells.参与O-连接寡糖合成的两种酶定位于小鼠淋巴瘤BW5147细胞中不同密度的膜上。
J Cell Biol. 1984 Jul;99(1 Pt 1):327-31. doi: 10.1083/jcb.99.1.327.
8
Subcellular distribution of UDP-galactose:ceramide galactosyltransferase in rat brain oligodendroglia.大鼠脑少突胶质细胞中UDP-半乳糖：神经酰胺半乳糖基转移酶的亚细胞分布
J Neurochem. 1988 Jun;50(6):1887-93. doi: 10.1111/j.1471-4159.1988.tb02493.x.
9
Characterization and membrane organization of beta 1----3- and beta 1----4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403: basis for preferential synthesis of type 1 chain lacto-series carbohydrate structures.
Arch Biochem Biophys. 1989 May 1;270(2):630-46. doi: 10.1016/0003-9861(89)90546-8.
10
UDP-galactose-ceramide galactosyltransferase in rat brain myelin subfractions during development.发育过程中大鼠脑髓磷脂亚组分中的UDP-半乳糖-神经酰胺半乳糖基转移酶
Biochem J. 1980 Mar 15;186(3):959-69. doi: 10.1042/bj1860959.
引用本文的文献
1
Biochemical and physiological characterization of fut4 and fut6 mutants defective in arabinogalactan-protein fucosylation in Arabidopsis.拟南芥中缺失岩藻糖基转移酶 Fut4 和 Fut6 导致阿拉伯半乳聚糖蛋白糖基化缺陷的生化和生理学特征。
J Exp Bot. 2013 Dec;64(18):5537-51. doi: 10.1093/jxb/ert321. Epub 2013 Oct 14.
2
Arabinogalactan-proteins and the research challenges for these enigmatic plant cell surface proteoglycans.阿拉伯半乳聚糖蛋白和这些神秘的植物细胞表面蛋白聚糖的研究挑战。
Front Plant Sci. 2012 Jun 27;3:140. doi: 10.3389/fpls.2012.00140. eCollection 2012.
3
Identification and characterization of in vitro galactosyltransferase activities involved in arabinogalactan-protein glycosylation in tobacco and Arabidopsis.鉴定和表征体外半乳糖基转移酶在烟草和拟南芥阿拉伯半乳聚糖蛋白糖基化中的活性。
Plant Physiol. 2010 Oct;154(2):632-42. doi: 10.1104/pp.110.160051. Epub 2010 Jul 29.
4
Arabinogalactan-proteins: key regulators at the cell surface?阿拉伯半乳聚糖蛋白：细胞表面的关键调节因子？
Plant Physiol. 2010 Jun;153(2):403-19. doi: 10.1104/pp.110.156000. Epub 2010 Apr 13.
5
Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases.一组新的拟南芥假定β-(1,3)-半乳糖基转移酶的鉴定。
Plant Mol Biol. 2008 Sep;68(1-2):43-59. doi: 10.1007/s11103-008-9351-3. Epub 2008 Jun 12.
6
In vitro biosynthesis of galactans by membrane-bound galactosyltransferase from radish ( Raphanus sativus L.) seedlings.萝卜（Raphanus sativus L.）幼苗中膜结合半乳糖基转移酶体外生物合成半乳聚糖
Planta. 2003 Jun;217(2):271-82. doi: 10.1007/s00425-003-0978-7. Epub 2003 Feb 11.
本文引用的文献
1
Biosynthesis of Arabinogalactan-Protein in Lolium multiflorum (Ryegrass) Endosperm Cells : III. Subcellular Distribution of Prolyl Hydroxylase.多花黑麦草（黑麦草）胚乳细胞中阿拉伯半乳聚糖蛋白的生物合成：III. 脯氨酰羟化酶的亚细胞分布
Plant Physiol. 1983 Jul;72(3):754-8. doi: 10.1104/pp.72.3.754.
2
ISOLATION OF beta-GLUCAN SYNTHETASE PARTICLES FROM PLANT CELLS AND IDENTIFICATION WITH GOLGI MEMBRANES.从植物细胞中分离β-葡聚糖合成酶颗粒并与高尔基体膜进行鉴定。
Proc Natl Acad Sci U S A. 1969 Oct;64(2):605-12. doi: 10.1073/pnas.64.2.605.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Elimination of thiol reagent interference during Lowry protein determination.
Anal Biochem. 1981 Oct;117(1):1-5. doi: 10.1016/0003-2697(81)90681-3.
5
Isolation of plasma membrane from protoplasts of Lolium multiflorum (ryegrass) endosperm cells.从多花黑麦草（黑麦草）胚乳细胞原生质体中分离质膜
Biochem J. 1982 Sep 1;205(3):511-9. doi: 10.1042/bj2050511.
6
Galactose transfer to endogenous acceptors within Golgi fractions of rat liver.半乳糖向大鼠肝脏高尔基体部分内源性受体的转移。
J Cell Biol. 1982 Jan;92(1):139-46. doi: 10.1083/jcb.92.1.139.
7
The location of arabinosyl:hydroxyproline transferase in the membrane system of potato tissue culture cells.阿拉伯糖基：羟脯氨酸转移酶在马铃薯组织培养细胞内膜系统中的定位。
Biochem J. 1981 Jun 1;195(3):661-7. doi: 10.1042/bj1950661.
8
Subfractionation of rat liver Golgi apparatus: separation of enzyme activities involved in the biosynthesis of the phosphomannosyl recognition marker in lysosomal enzymes.大鼠肝脏高尔基体的亚分级分离：溶酶体酶中磷酸甘露糖识别标记生物合成相关酶活性的分离
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3938-42. doi: 10.1073/pnas.80.13.3938.
9
Localization of beta-glucan synthases on the membranes of cultured Lolium multiflorum (ryegrass) endosperm cells.β-葡聚糖合酶在多花黑麦草（黑麦草）胚乳细胞培养膜上的定位。
Biochem J. 1983 Mar 1;209(3):627-33. doi: 10.1042/bj2090627.
10
Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.从大鼠肝脏匀浆中制备的高尔基体组分。I. 分离程序和形态学特征。
J Cell Biol. 1973 Oct;59(1):45-72. doi: 10.1083/jcb.59.1.45.
Zotero 插件