Chronic regulation by thyrotropin of arachidonic acid incorporation in cholesteryl esters of cultured thyroid cells.

During short term incubations, radioactive arachidonic acid and palmitic acid were incorporated in the cholesteryl ester fraction of the lipids of cultured thyroid cells. Three times more arachidonic than palmitic acid was incorporated and the incorporation of both was dependent upon the culture conditions: the presence of 1 mU/ml thyrotropin in the culture medium during four days almost completely inhibited the subsequent incorporation of the two fatty acids in the cholesteryl ester fraction whereas the total cholesterol and cholesteryl ester content of the cells was not affected.