Iritani N, Narita R
Biochim Biophys Acta. 1984 May 11;793(3):441-7.
When rats adapted to a fat-free diet were fed a corn oil diet, endogenous n-9 eicosatrienoic acid (the major polyunsaturated fatty acid) at the C-2 position of both phosphatidylcholine and phosphatidylethanolamine was quickly substituted by arachidonic acid in liver, plasma and platelets. Comparably, under a fish oil diet, the n-9 was quickly substituted by n-3 polyunsaturated fatty acids (eicosapentaenoic acid and docosahexaenoic acid). In both cases the n-9 almost disappeared in 6 days. On the other hand, when the dietary process was reversed, arachidonic acid in both the phospholipid classes (especially in phosphatidylcholine) decreased more slowly than the n-3 in the platelets and the liver mitochondria and microsomes. In platelets, even in linoleate-deficient rats, much arachidonic acid remained. However, arachidonic acid decreased similarly to the n-3 in the plasma. These results may reveal the physiological significance of arachidonic acid in membrane phospholipids, the replacement of arachidonic acid by the n-3 and the limitation of the replacement.
当适应无脂饮食的大鼠改喂玉米油饮食时，肝脏、血浆和血小板中磷脂酰胆碱和磷脂酰乙醇胺C-2位的内源性n-9二十碳三烯酸（主要的多不饱和脂肪酸）迅速被花生四烯酸取代。同样，在喂鱼油饮食的情况下，n-9迅速被n-3多不饱和脂肪酸（二十碳五烯酸和二十二碳六烯酸）取代。在这两种情况下，n-9在6天内几乎消失。另一方面，当饮食过程逆转时，两种磷脂类（尤其是磷脂酰胆碱）中的花生四烯酸减少得比血小板以及肝脏线粒体和微粒体中的n-3更慢。在血小板中，即使是缺乏亚油酸的大鼠，仍保留有大量花生四烯酸。然而，血浆中花生四烯酸的减少情况与n-3相似。这些结果可能揭示了膜磷脂中花生四烯酸的生理意义、n-3对花生四烯酸的取代以及这种取代的局限性。
