Fesmire J D, McConathy W J, Alaupovic P
Clin Chem. 1984 May;30(5):712-6.
This study was designed to show the effect of different primary standards and antisera on the variability of concentrations of apolipoprotein A-I (ApoA-I) in plasma and to document the usefulness of reference sera as a secondary standard in the electroimmunoassay of this apolipoprotein. We compared the reactivities of two separate ApoA-I samples and a preparation of high-density lipoprotein (HDL3) with seven different antisera to ApoA-I. The antisera reacted differently with each of the three standards, as shown by the different slopes of their standard curves. The resulting inconsistencies in the ApoA-I values for unknown plasma samples were shown to be corrected by the use of a reference serum. When ApoA-I analyses were carried out with the reference serum as a standard, all seven antisera gave statistically indistinguishable results for concentrations of plasma ApoA-I. The use of reference sera thus represents the simplest and most convenient means of standardizing the electroimmunoassay for ApoA-I.
本研究旨在展示不同一级标准品和抗血清对血浆中载脂蛋白A-I（ApoA-I）浓度变异性的影响，并记录参考血清作为该载脂蛋白免疫电泳分析中二级标准品的实用性。我们比较了两个单独的ApoA-I样本以及一种高密度脂蛋白（HDL3）制剂与七种不同抗ApoA-I血清的反应性。抗血清与三种标准品中的每一种反应不同，这从它们标准曲线的不同斜率可以看出。结果表明，使用参考血清可纠正未知血浆样本ApoA-I值中出现的不一致情况。当以参考血清作为标准进行ApoA-I分析时，所有七种抗血清对血浆ApoA-I浓度给出的结果在统计学上无显著差异。因此，使用参考血清是使ApoA-I免疫电泳分析标准化的最简单、最方便的方法。
