Magnusson C G, Delacroix D L, Vaerman J P, Masson P L
J Immunol Methods. 1984 Apr 27;69(2):229-41. doi: 10.1016/0022-1759(84)90321-1.
The subclasses of monoclonal IgGs and IgAs were identified by particle-counting immunoassay. The principle of the test is the inhibition of the agglutinating activity of either specific antisera or monoclonal antibodies (for IgA only) on latex particles coated with a monoclonal IgG or IgA of known subclass. The feasibility of assay of polyclonal Ig subclasses was demonstrated. However, the anti-IgG2 antiserum cross-reacted with an allotype (nG4m(b)) of IgG4. The possibility of typing monoclonal Igs for light chains by the same technique was also demonstrated. Results are obtained in 30 min, and the method requires only small amounts of purified immunoglobulins (Igs) and antisera or monoclonal antibodies.
通过颗粒计数免疫测定法鉴定单克隆IgG和IgA的亚类。该检测原理是抑制特异性抗血清或单克隆抗体（仅针对IgA）对包被有已知亚类单克隆IgG或IgA的乳胶颗粒的凝集活性。证明了检测多克隆Ig亚类的可行性。然而，抗IgG2抗血清与IgG4的一种同种异型（nG4m(b)）发生交叉反应。还证明了用相同技术对单克隆Ig轻链进行分型的可能性。30分钟内即可获得结果，该方法仅需要少量纯化的免疫球蛋白（Ig）和抗血清或单克隆抗体。
