Wietzerbin J, Kolb J P, Senik A, Der Stepani L, Andreu G, Falcoff E, Falcoff R
J Interferon Res. 1984;4(1):141-52. doi: 10.1089/jir.1984.4.141.
Gamma interferon (gamma IFN) was produced in human lymphocyte cultures stimulated by PHA. Titers were in the range of 10,000-30,000 U/ml. Crude gamma IFN was adsorbed on silicic acid, from which the antiviral activity was eluted by a buffer containing a high salt concentration and ethylene glycol. This treatment allowed quantitative recovery of gamma IFN with a specific activity of 5 X 10(5)-1 X 10(6) U/mg of proteins. IL2 and B-cell helper activities were adsorbed and eluted from silicic acid together with the antiviral activity. This finding might be of practical interest for the purification of these lymphokines, particularly IL2. Gamma interferon was further purified on Blue Sepharose to a specific activity of 2 X 10(7) U/mg. The resulting preparations still contained IL2 and B cell helper activities. However, taking advantage of the differences in apparent hydrophobicity and in isoelectric point, we were able to dissociate antiviral activity from lymphokines. Such dissociation should facilitate the study of the biological properties of human natural gamma IFN.
γ干扰素（γ-IFN）是在由PHA刺激的人淋巴细胞培养物中产生的。效价在10,000 - 30,000 U/ml范围内。粗制的γ-IFN吸附在硅酸上，其抗病毒活性通过含有高盐浓度和乙二醇的缓冲液洗脱。这种处理使得γ-IFN能够定量回收，比活性为5×10⁵ - 1×10⁶ U/mg蛋白质。IL2和B细胞辅助活性与抗病毒活性一起从硅酸上吸附和洗脱。这一发现对于这些淋巴因子，特别是IL2的纯化可能具有实际意义。γ干扰素在蓝色琼脂糖凝胶上进一步纯化至比活性为2×10⁷ U/mg。所得制剂仍含有IL2和B细胞辅助活性。然而，利用明显的疏水性和等电点的差异，我们能够将抗病毒活性与淋巴因子解离。这种解离应有助于对人天然γ-IFN生物学特性的研究。
