Chiba J, Yamauchi H, Miyagawa K, Kakizaki K
Nihon Geka Gakkai Zasshi. 1983 Mar;84(3):245-55.
A reproducible model of a diabetic state was produced after resecting more than 90% of pancreatic tissue in Wistar strain male rat (outbred and inbred group). Of a total of 10 animals of outbred group, 6 animals illustrated a blood glucose level over 250 mg/dl in an earlier stage after an injection of 1500 islets into the portal vein. However, in 4 of them blood glucose levels were maintained within normal limit until 4 months. On the other hand, 5 inbred rats in which 500-600 islets were injected demonstrated reversal of diabetes within 1 week after the injection and the effect of the transplantation lasted until about 6 months. A morphometric analysis using 1190 serial histological sections of the control rat pancreas showed that the islet distribution accorded satisfactorily with Weibull or Gamma distribution. The estimated values of size and number of the islet were 0.0155 mm and 131.2/mm3, respectively, while volume ratio of splenic and gastric segments or parabiliary and duodenal segments was estimated to be 1.45 or 0.98%, respectively. The mean size of the isolated islets under dissecting microscope reached 0.080 mm and 500 of them were equivalent to about 10% volume fraction in a whole control pancreas. In the inbred group a transplanted animal contained about 17% of islet volume as compared with control animal.
在切除Wistar品系雄性大鼠（远交系和近交系组）90%以上的胰腺组织后，建立了可重复的糖尿病状态模型。在远交组的10只动物中，有6只在门静脉注射1500个胰岛后的早期血糖水平超过250mg/dl。然而，其中4只动物的血糖水平在4个月内一直维持在正常范围内。另一方面，注射500 - 600个胰岛的5只近交系大鼠在注射后1周内糖尿病症状逆转，移植效果持续到约6个月。对对照大鼠胰腺的1190个连续组织学切片进行形态计量分析表明，胰岛分布符合威布尔分布或伽马分布。胰岛大小和数量的估计值分别为0.0155mm和131.2/mm³，而脾段和胃段或胆管旁段和十二指肠段的体积比估计分别为1.45%或0.98%。在解剖显微镜下分离的胰岛平均大小达到0.080mm，500个这样的胰岛相当于整个对照胰腺约10%的体积分数。在近交系组中，与对照动物相比，移植动物的胰岛体积约占17%。
