Strömberg I, Herrera-Marschitz M, Hultgren L, Ungerstedt U, Olson L
Brain Res. 1984 Apr 9;297(1):41-51. doi: 10.1016/0006-8993(84)90541-9.
Rats with unilateral 6-hydroxydopamine-induced degeneration of the left nigrostriatal dopamine system were given intrastriatal implants of one cortex-free adrenal medulla divided into 4 pieces. Two pieces were placed in the center of the anterior part of the denervated caudate and two pieces in a more posterior position in lateral caudate. The distribution of catecholamines (CA) in grafts and host brain was studied 2, 100 and 400 min after grafting by HPLC-electrochemistry and Falck-Hillarp fluorescence histochemistry combined with computer-aided image analysis. Two minutes after implantation the chromaffin tissue grafts contained large amounts of adrenaline (A) and noradrenaline (NA) and small amounts of dopamine (DA). The chromaffin cells had a relatively normal fluorescence histochemical appearance. From the grafts, CA had spread into the surrounding host brain tissue where high levels of A and NA and low levels of DA were now found in the denervated host striatum. Fluorescence histochemistry and image analysis showed the CA to have spread 1-1.5 mm in all directions from the grafts. The CA concentrations decreased almost linearly with increasing distance from the grafts. At 100 min after implantation approximately a third of the chromaffin cells were still strongly fluorescent while the rest of the cells were very weakly fluorescent or non-fluorescent. The amounts of A, NA and DA in the host brain had decreased considerably, while the size of the fluorescent halo around the grafts had not diminished. At 400 min after grafting, only scattered cells in the chromaffin implants were strongly fluorescent and the surrounding host striatum contained low amounts of CA. It is concluded that intrastriatal adrenal medullary implants acutely release or leak large amounts of CA into surrounding host brain tissue. Taken together with results from the accompanying paper these data show that the grafts can maintain CA levels in host striatum high enough to elicit strong rotational responses during approximately 200 min.
对单侧6-羟基多巴胺诱导左黑质纹状体多巴胺系统变性的大鼠，将一个无皮质的肾上腺髓质切成4片后植入纹状体。两片置于去神经尾状核前部中央，两片置于外侧尾状核更靠后的位置。移植后2分钟、100分钟和400分钟，通过高效液相色谱-电化学法以及福尔克-希拉尔普荧光组织化学结合计算机辅助图像分析，研究移植物和宿主脑内儿茶酚胺（CA）的分布。植入后2分钟，嗜铬组织移植物含有大量肾上腺素（A）和去甲肾上腺素（NA）以及少量多巴胺（DA）。嗜铬细胞具有相对正常的荧光组织化学外观。CA从移植物扩散到周围宿主脑组织，在去神经的宿主纹状体内发现高水平的A和NA以及低水平的DA。荧光组织化学和图像分析显示，CA从移植物向各个方向扩散了1 - 1.5毫米。CA浓度几乎随距移植物距离的增加呈线性下降。植入后100分钟，约三分之一的嗜铬细胞仍强烈荧光，其余细胞荧光非常弱或无荧光。宿主脑内A、NA和DA的量已大幅下降，而移植物周围荧光晕的大小并未减小。移植后400分钟，嗜铬植入物中只有散在细胞强烈荧光，周围宿主纹状体含有少量CA。得出的结论是，纹状体内肾上腺髓质植入物会急性释放或向周围宿主脑组织渗漏大量CA。结合随附论文的结果，这些数据表明移植物可使宿主纹状体内CA水平维持足够高，以在约200分钟内引发强烈的旋转反应。
