Liav A, Goren M B
Carbohydr Res. 1984 Apr 15;127(2):211-6. doi: 10.1016/0008-6215(84)85355-0.
alpha,alpha-Trehalose 2-sulfate, the core carbohydrate of sulfatides of Mycobacterium tuberculosis, and the 3-sulfate isomer were synthesized by sulfation of 4,6:4',6'-di-O-benzylidene-alpha,alpha-trehalose with pyridine-sulfur trioxide complex to give the 2- and 3-sulfates, which were separated by column chromatography. The ammonium 2-sulfate salt wa was identical with the natural product obtained from the principal sulfatide (SL-I) of M. tuberculosis.
α,α-海藻糖2-硫酸盐，即结核分枝杆菌硫脂的核心碳水化合物，以及3-硫酸盐异构体，是通过用吡啶-三氧化硫络合物对4,6:4',6'-二-O-亚苄基-α,α-海藻糖进行硫酸化反应生成2-硫酸盐和3-硫酸盐，然后通过柱色谱法将它们分离得到的。2-硫酸盐铵盐与从结核分枝杆菌主要硫脂（SL-I）中获得的天然产物相同。
