Nakhasi H L, Nagarajan L, Anderson W B
FEBS Lett. 1984 Mar 26;168(2):222-6. doi: 10.1016/0014-5793(84)80250-1.
Exposure of F9 cells to all-trans-retinoic acid over a period of 6 days resulted in 4-fold induction of cell surface N-acetylglucosaminide beta (1----4)galactosyltransferase (GT) activity. The retinoic acid-induced GT activity was further enhanced by treatment of the cells with 8-bromo cyclic AMP. The ability of retinoic acid alone, or retinoic acid in combination with 8-bromo cyclic AMP, to induce GT activity was inhibited by both actinomycin D and cycloheximide. These findings indicate that the induction of galactosyltransferase activity noted with differentiation of F9 cells involves de novo synthesis of new enzyme protein.
将F9细胞暴露于全反式维甲酸6天，可使细胞表面N-乙酰葡糖胺β(1→4)半乳糖基转移酶(GT)活性诱导增加4倍。用8-溴环磷酸腺苷处理细胞可进一步增强维甲酸诱导的GT活性。放线菌素D和环己酰亚胺均可抑制单独使用维甲酸或维甲酸与8-溴环磷酸腺苷联合使用诱导GT活性的能力。这些发现表明，F9细胞分化时观察到的半乳糖基转移酶活性的诱导涉及新酶蛋白的从头合成。
