Stouffer R L, Hodgen G D, Ottobre A C, Christian C D
J Clin Endocrinol Metab. 1984 Jun;58(6):1027-33. doi: 10.1210/jcem-58-6-1027.
Administration of charcoal-extracted porcine follicular fluid (pFF) to rhesus monkeys at the time of menses impairs the subsequent function of the corpus luteum of the menstrual cycle. The following studies were performed: 1) to characterize the luteal phase defect induced by pFF treatment at menses, and 2) to determine whether pFF treatment in the luteal phase alters corpus luteum function. Adult, female rhesus monkeys were injected sc for 3 days with pFF (10, 5, and 5 ml) beginning on day 1 (n = 5) or day 18 (n = 4) of the menstrual cycle. Femoral venous blood was collected daily throughout the treatment cycle and during the posttreatment cycle of day 18 to 20-treated monkeys. Serum LH, FSH, 17 beta-estradiol (E2), and progesterone (P) were measured by RIA. After pFF treatment on days 1-3, FSH and E2 levels in the early follicular phase were less (P less than 0.05) than those of control cycles (n = 7). Serum LH was not suppressed by pFF treatment. Moreover, the preovulatory rise in circulating E2 and the amplitude of the LH/FSH surge were similar in control and pFF-treated monkeys. Although timely midcycle gonadotropin surges occurred in four of five pFF-treated monkeys, serum P was markedly reduced (P less than 0.05) during the first half of the luteal phase. Circulating P increased to control levels during the late luteal phase before normal onset of menses 16.3 +/- 1.0 (SE) days after the LH surge. Treatment with pFF on days 18-20 of the cycle reduced the levels of circulating FSH, but serum LH, E2, P, and the length of the luteal phase remained comparable to control cycles. Moreover, the hormonal patterns and the length of the follicular and luteal phases in the posttreatment cycle indicated normal ovarian function. Thus, pFF treatment at menses results in an aberrant ovarian cycle characterized by an insufficient, rather than short, luteal phase. Whereas pFF treatment in the early follicular phase vitiates development of the dominant follicle and the related corpus luteum, similar treatment at midluteal phase does not suppress concurrent luteal function or subsequent folliculogenesis.
在月经期间给恒河猴注射经活性炭提取的猪卵泡液（pFF）会损害随后月经周期中黄体的功能。进行了以下研究：1）描述月经时pFF处理诱导的黄体期缺陷，以及2）确定黄体期的pFF处理是否会改变黄体功能。成年雌性恒河猴在月经周期的第1天（n = 5）或第18天（n = 4）开始皮下注射pFF（10、5和5毫升），持续3天。在整个治疗周期以及第18至20天接受治疗的猴子的治疗后周期中，每天采集股静脉血。通过放射免疫分析法测定血清促黄体生成素（LH）、促卵泡生成素（FSH）、17β-雌二醇（E2）和孕酮（P）。在第1 - 3天进行pFF处理后，卵泡早期的FSH和E2水平低于（P < 0.05）对照周期（n = 7）。pFF处理未抑制血清LH。此外，对照猴和接受pFF处理的猴中，循环E2的排卵前升高以及LH/FSH峰的幅度相似。尽管五只接受pFF处理的猴子中有四只在周期中期出现了适时的促性腺激素峰，但黄体期前半期的血清P显著降低（P < 0.05）。在LH峰后16.3±1.0（SE）天正常月经开始前的黄体晚期，循环P升高至对照水平。在周期的第18 - 20天用pFF处理可降低循环FSH水平，但血清LH、E2、P以及黄体期长度与对照周期相当。此外，治疗后周期中的激素模式以及卵泡期和黄体期的长度表明卵巢功能正常。因此，月经时的pFF处理导致异常的卵巢周期，其特征是黄体期不足而非短暂。卵泡早期的pFF处理会损害优势卵泡和相关黄体的发育，而黄体中期的类似处理不会抑制同期黄体功能或随后的卵泡生成。
