Lorian V, Atkinson B
J Infect Dis. 1984 May;149(5):719-27. doi: 10.1093/infdis/149.5.719.
Exposure of strains of Escherichia coli to ampicillin and mezlocillin and of strains of Pseudomonas aeruginosa to azlocillin and cefsulodin caused the bacilli to elongate into filaments. The bacilli and their filaments were incubated with fresh human polymorphonuclear neutrophils (PMNs), and the phagocytic process was recorded by means of phase-contrast microscopy. The bactericidal effect of PMNs on both filaments and bacilli was quantitated by counts of colony-forming units. A single PMN phagocytized one or more filaments, some of which were as long as 90 micron and contained as many as 20 genomes. Two predominant patterns of phagocytosis of filaments were observed. When the ratio of bacteria to PMNs was low (0.2-1.8), the rate of killing was 62%-81%. When the ratio was higher (5-12), the rate of killing of both filaments and bacilli was lower. As an alternative to colony-forming units, cell mass was used as a gauge of phagocytic activity. The relative mass of killed filaments was considerably greater than that of killed bacilli; this finding indicated that filaments were much more susceptible than were bacilli to the bactericidal activity of PMNs.
将大肠杆菌菌株暴露于氨苄青霉素和美洛西林，以及将铜绿假单胞菌菌株暴露于阿洛西林和头孢磺啶，会导致杆菌伸长为细丝状。将这些杆菌及其细丝与新鲜的人多形核中性粒细胞（PMN）一起孵育，并通过相差显微镜记录吞噬过程。通过菌落形成单位计数来定量PMN对细丝和杆菌的杀菌作用。单个PMN吞噬一根或多根细丝，其中一些细丝长达90微米，包含多达20个基因组。观察到两种主要的细丝吞噬模式。当细菌与PMN的比例较低（0.2 - 1.8）时，杀菌率为62% - 81%。当比例较高（5 - 12）时，细丝和杆菌的杀菌率都较低。作为菌落形成单位的替代方法，细胞质量被用作吞噬活性的指标。被杀死细丝的相对质量明显大于被杀死杆菌的相对质量；这一发现表明，细丝比杆菌对PMN的杀菌活性更敏感。
