采用单向放射免疫扩散法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对混合人血清中的载脂蛋白A-I进行定量分析。
Quantitation of apolipoprotein A-I in pooled human serum by single radial immunodiffusion and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
作者信息
Sakai Y, Itakura K, Kanada T, Ebata N, Suga K, Aikawa H, Nakamura K, Sata T
出版信息
Anal Biochem. 1984 Feb;137(1):1-7. doi: 10.1016/0003-2697(84)90337-3.
PMID:6428261
Abstract
Apolipoprotein A-I was released from human HDL particles by treatment with 8 M urea, and the free apolipoprotein exhibited identical antigenicity and the same low mobility as purified apolipoprotein A-I in electrophoresis. Treatment of serum with 8 M urea enabled enabled quantitation of apolipoprotein A-I by single radial immunodiffusion assay, as judged by comparison with sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
摘要
用8M尿素处理人高密度脂蛋白（HDL）颗粒可释放载脂蛋白A-I，在电泳中，游离的载脂蛋白表现出与纯化的载脂蛋白A-I相同的抗原性和相同的低迁移率。通过与十二烷基硫酸钠-聚丙烯酰胺凝胶电泳比较判断，用8M尿素处理血清能够通过单向放射免疫扩散试验对载脂蛋白A-I进行定量。
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