通过在三层氯化铯梯度中利用浮力密度快速分离DNA。
Rapid separation of DNAs by buoyant density in three-layer CsCl gradients.
作者信息
Babykin M M, Zinchenko V V
出版信息
Anal Biochem. 1984 Feb;137(1):175-81. doi: 10.1016/0003-2697(84)90367-1.
PMID:6428263
Abstract
The formation of three-layer CsCl gradients with a narrow middle layer containing the analyzed material allows the separation of macrospecies according to their buoyant densities during the first hours of centrifugation before equilibrium is attained. The method can be used for the isolation and purification of various DNA molecules, such as mitochondrial and plasmid DNAs.
摘要
形成具有包含被分析物质的狭窄中间层的三层氯化铯梯度，能够在达到平衡之前的离心最初几小时内，根据大分子物质的浮力密度对其进行分离。该方法可用于分离和纯化各种DNA分子，如线粒体DNA和质粒DNA。
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