Dieguez C, Foord S M, Shewring G, Edwards C A, Heyburn P J, Peters J R, Hall R, Scanlon M F
Biochem Biophys Res Commun. 1984 May 31;121(1):111-7. doi: 10.1016/0006-291x(84)90694-6.
The ability of human pancreatic GH releasing factor 1-40 (hpGRF 1-40) to release GH has been studied in rat anterior pituitary cells in primary culture. Over 24 hours hpGRF (1-40) increased total (cell content and secretion) production 2-fold with an ED50 of 20 pM. Subsequent hpGRF (1-40) stimulation of GH release was not affected by pretreatment when the fall in stored GH was taken into account. In contrast LH responses to gonadotrophin releasing hormone (GnRH) were markedly desensitized after 24 hours GnRH pretreatment in the same experimental system and using the same analysis. hpGRF (1-40) responses were not desensitized when pretreatment was for 3, 12 or 24 hours. The data show that hpGRF (1-40) responses do not desensitize in our experimental conditions under which GnRH responses show marked desensitization.
在原代培养的大鼠垂体前叶细胞中，对人胰腺生长激素释放因子1 - 40（hpGRF 1 - 40）释放生长激素（GH）的能力进行了研究。在24小时内，hpGRF（1 - 40）使总（细胞内含量和分泌量）产量增加了2倍，半数有效剂量（ED50）为20皮摩尔。当考虑到储存的GH下降时，随后hpGRF（1 - 40）对GH释放的刺激不受预处理的影响。相比之下，在相同的实验系统中，使用相同的分析方法，在促性腺激素释放激素（GnRH）预处理24小时后，促黄体生成素（LH）对GnRH的反应明显脱敏。当预处理时间为3小时、12小时或24小时时，hpGRF（1 - 40）的反应并未脱敏。数据表明，在我们的实验条件下，GnRH反应出现明显脱敏时，hpGRF（1 - 40）的反应不会脱敏。
