HayGlass K T, Strejan G H
Int Arch Allergy Appl Immunol. 1984;74(4):332-40. doi: 10.1159/000233569.
Ovalbumin (OA) was substituted with glutaraldehyde (GA) at various GA:OA ratios and several preparations were isolated by gel filtration according to molecular weight. Two GA-substituted but unpolymerized preparations (OA1-L and OA1-H) and 3 polymerized preparations of increasing molecular weight (OA4, OA175 and OA-POL) were obtained and were assessed for their ability to react with IgG and IgE antibodies directed against the native OA molecule, as well as for their ability to suppress the IgE antibody response. The results show that while both polymerized and unpolymerized GA-modified preparations lost their reactivity towards antinative OA antibodies, to a considerable extent, their ability to suppress the OA/IgE antibody response was directly dependent on polymerization. Pretreatment of CBA mice with unpolymerized OA preparations was ineffective while the effect of pretreatment with three polymers (OA4, OA175 and OA-POL) was directly dependent on the size of the polymer. The ability to induce IgG antibodies or delayed hypersensitivity to OA was not suppressed.
用戊二醛（GA）以不同的GA:卵清蛋白（OA）比例替代卵清蛋白，并根据分子量通过凝胶过滤分离出几种制剂。获得了两种GA替代但未聚合的制剂（OA1-L和OA1-H）以及3种分子量递增的聚合制剂（OA4、OA175和OA-POL），并评估了它们与针对天然OA分子的IgG和IgE抗体反应的能力，以及它们抑制IgE抗体反应的能力。结果表明，虽然聚合和未聚合的GA修饰制剂在很大程度上都失去了与抗天然OA抗体的反应性，但其抑制OA/IgE抗体反应的能力直接取决于聚合作用。用未聚合的OA制剂预处理CBA小鼠无效，而用三种聚合物（OA4、OA175和OA-POL）预处理的效果直接取决于聚合物的大小。诱导IgG抗体或对OA的迟发型超敏反应的能力未被抑制。
