Sato M, Yoshida H, Urata M, Yanagawa T, Yura Y, Nitta T, Kobayashi S, Hayashi Y
Int J Oral Surg. 1984 Feb;13(1):35-44. doi: 10.1016/s0300-9785(84)80054-x.
The growth inhibitory effects of 5-fluorouracil (5-Fu) and the combination of 5-Fu and human leukocyte interferon (HuIFN-alpha) on the human salivary gland adenocarcinoma cell line HSG in culture were examined by measuring colony formation in the semi-solid agar medium and cell proliferation in the monolayer culture. As a consequence, the colony-forming ability of HSG cells in the agar medium containing 5-Fu was found to be markedly inhibited as compared with the untreated control. The concentration of 5-Fu yielding 50% inhibition of colony-forming ability of HSG cells under the presence of 5-Fu was 0.08 micrograms/ml. When the growth inhibitory effects of the combination of 5-Fu and HuIFN-alpha on HSG cells were examined, the colony forming ability of HSG cells was synergistically inhibited, whereas effects of the combined treatment on HSG cells in the monolayer culture were less sensitive than those on the colony formation. These findings strongly suggest that the combination of 5-Fu and HuIFN-alpha or 5-Fu alone is selectively effective on the neoplastic cells of HSG cell population but not on the non-neoplastic cells.
通过在半固体琼脂培养基中测量集落形成以及在单层培养中测量细胞增殖，研究了5-氟尿嘧啶（5-Fu）以及5-Fu与人白细胞干扰素（HuIFN-α）联合使用对培养的人涎腺腺癌细胞系HSG的生长抑制作用。结果发现，与未处理的对照相比，含5-Fu的琼脂培养基中HSG细胞的集落形成能力受到明显抑制。在5-Fu存在下，使HSG细胞集落形成能力受到50%抑制的5-Fu浓度为0.08微克/毫升。当检测5-Fu与HuIFN-α联合使用对HSG细胞的生长抑制作用时，HSG细胞的集落形成能力受到协同抑制，而联合处理对单层培养中HSG细胞的作用比对集落形成的作用敏感性更低。这些发现强烈表明，5-Fu与HuIFN-α联合使用或单独使用5-Fu对HSG细胞群体中的肿瘤细胞具有选择性作用，而对非肿瘤细胞则无作用。
