Leibson H J, Gefter M, Zlotnik A, Marrack P, Kappler J W
Nature. 1984;309(5971):799-801. doi: 10.1038/309799a0.
Interferon preparations, especially those containing gamma-interferon (IFN-gamma), have long been known to modulate immune responses. However, because many studies used only partially purified interferons, it has been difficult to separate the immunoregulatory effects of the interferons from those of other biologically active molecules contaminating the preparations. Recently, with the cloning of the interferon genes in mouse and man, it has become possible to use these cloned interferons directly to test their effects in assays other than those involving the protection of cells from viruses. For example, cloned IFN-gamma has been shown to be a potent inducer of Ia antigen expression on macrophages. Similarly, cloned IFN-gamma has been reported to act as a macrophage activation factors, as judged by the ability of activated macrophages to kill tumour cells in vitro. We demonstrate here that cloned murine IFN-gamma can also substitute for a late-acting helper factor which acts synergistically with other helper factors in the stimulation of B-cell antibody responses in vitro.
长期以来，人们一直知道干扰素制剂，尤其是那些含有γ-干扰素（IFN-γ）的制剂，能够调节免疫反应。然而，由于许多研究仅使用了部分纯化的干扰素，因此很难将干扰素的免疫调节作用与污染制剂的其他生物活性分子的作用区分开来。最近，随着小鼠和人类干扰素基因的克隆，直接使用这些克隆的干扰素在除涉及保护细胞免受病毒侵害之外的其他检测中测试其效果已成为可能。例如，已证明克隆的IFN-γ是巨噬细胞上Ia抗原表达的有效诱导剂。同样，据报道，克隆的IFN-γ可作为巨噬细胞激活因子，这是根据活化巨噬细胞在体外杀死肿瘤细胞的能力来判断的。我们在此证明，克隆的小鼠IFN-γ还可以替代一种晚期作用的辅助因子，该因子在体外刺激B细胞抗体反应时与其他辅助因子协同作用。
