化学诱导显性致死的研究。III. 透射电子显微镜对成熟双线期小鼠卵母细胞影响的细胞遗传学分析。

Studies on chemically induced dominant lethality. III. Cytogenetic analyses of TEM-effects on maturing dictyate mouse oocytes.

作者信息

Brewen J G, Payne H S

出版信息

Mutat Res. 1978 Apr;50(1):85-92. doi: 10.1016/0027-5107(78)90063-5.

Abstract

A comparative cytogenetic study was done on metaphase-I oocytes and first cleavage mitoses following treatment of young mature female mice with triethylenemelamine (TEM). The ova were collected at intervals ranging from 12 h to 10.5 days after single intraperitoneal injection of TEM. Very few structural aberrations were seen in the metaphase-I cells after TEM treatment. There was, however, a very clear effect on the female genome when first cleavage mitoses were analyzed. The aberrations seen were principally chromatid deletions and interchanges, and their frequency varied with dose and the time between treatment and mating.

摘要

用三亚乙基三聚氰胺（TEM）处理年轻成熟雌性小鼠后，对中期I卵母细胞和第一次卵裂有丝分裂进行了比较细胞遗传学研究。在单次腹腔注射TEM后12小时至10.5天的不同时间间隔收集卵子。TEM处理后，中期I细胞中很少见到结构畸变。然而，在分析第一次卵裂有丝分裂时，对雌性基因组有非常明显的影响。观察到的畸变主要是染色单体缺失和互换，其频率随剂量以及处理与交配之间的时间而变化。