公猪顶体蛋白酶原：总顶体蛋白酶原含量与精子受精能力之间的相关性。

Boar proacrosin correlation between total proacrosin content and sperm fertilizing capacity.

作者信息

Cechová D, Zelezná B, Petelíková J, Pavlok A

出版信息

Andrologia. 1984 Mar-Apr;16(2):169-74. doi: 10.1111/j.1439-0272.1984.tb00259.x.

DOI:10.1111/j.1439-0272.1984.tb00259.x

Abstract

A method of acidic extraction at pH-1.3 was used for quantitative estimation of the proacrosin and acrosin content in boar spermatozoa after various times of storage in four different sperm dilutors. The proacrosin and acrosin contents were correlated with the results of biological tests for sperm penetration in zona-free hamster eggs and sperm survival. The results have shown that proacrosin quantitation should be a convenient biochemical test of sperm fertilizing capacity.

摘要

采用pH值为1.3的酸性提取法，对在四种不同精子稀释液中储存不同时间后的公猪精子中的前顶体蛋白酶和顶体蛋白酶含量进行定量测定。前顶体蛋白酶和顶体蛋白酶含量与无透明带仓鼠卵精子穿透及精子存活的生物学测试结果相关。结果表明，前顶体蛋白酶定量测定应是一种方便的精子受精能力生化检测方法。