碳酸酐酶III的激素调控

Hormonal control of carbonic anhydrase III.

作者信息

Carter N D, Shiels A, Jeffery S, Heath R, Wilson C A, Phillips I R, Shephard E A

出版信息

Ann N Y Acad Sci. 1984;429:287-301. doi: 10.1111/j.1749-6632.1984.tb12352.x.

DOI:10.1111/j.1749-6632.1984.tb12352.x

Abstract

Using radioimmunoassay, the concentration of carbonic anhydrase III (CA III) in the livers of adult male rats was found to be approximately 30 times greater than that observed in mature females. Castration of male rats led to a marked reduction in liver CA III concentrations that could be partially restored to control levels by testosterone replacement. Administration of testosterone to ovariectomized female rats induced about a 5-fold increase in liver CA III concentration. Immunoprecipitational analysis of the products of liver mRNA translation in vitro with antiserum specific for CA III showed that hormonal control of the levels of CA III in rat liver is mediated by changes in the amount of translatable CA III mRNA. Marked changes in liver CA III concentrations were also observed in developing and aging male rats. Different control mechanisms appear to operate in mouse and man.

摘要

采用放射免疫分析法发现，成年雄性大鼠肝脏中碳酸酐酶III（CA III）的浓度约为成熟雌性大鼠的30倍。雄性大鼠去势后，肝脏CA III浓度显著降低，而通过补充睾酮可使其部分恢复至对照水平。给去卵巢雌性大鼠注射睾酮可使肝脏CA III浓度增加约5倍。用针对CA III的抗血清对体外肝脏mRNA翻译产物进行免疫沉淀分析表明，大鼠肝脏中CA III水平的激素调控是由可翻译的CA III mRNA量的变化介导的。在发育中和衰老的雄性大鼠中也观察到肝脏CA III浓度的显著变化。小鼠和人类似乎存在不同的调控机制。