Lang E, Mark D, Miller F A, Miller D, Wik O
Arch Ophthalmol. 1984 Jul;102(7):1079-82. doi: 10.1001/archopht.1984.01040030873037.
In this study, methods were developed for the in vitro evaluation of the surgical performance characteristics of viscoelastic fluids, such as sodium hyaluronate (Healon). Sodium hyaluronate exhibited superior surgical performance to chondroitin-6-sulfate. The superior performance of sodium hyaluronate resulted in part from its high viscosity, which is 20 times greater than that of chondroitin-6-sulfate at shear rates on the order of 10 s-1. The gel-like character of sodium hyaluronate as evidenced by the creep flow behavior, was greater than that of chondroitin-6-sulfate and was important for maintaining depth in the anterior chamber. It was shown that a threshold of 80 poise for the shear viscosity (at approximately 10 s-1 was needed for useful performance in surgery. It was also shown that 0.42 USP units of hyaluronidase per 1.0 mg sodium hyaluronate produces a 90% decrease in the shear viscosity (at approximately 10 s-1) within approximately 2 1/2 hours. Use of sodium hyaluronate in conjunction with hyaluronidase would allow sodium hyaluronate to remain highly viscous during surgery, but would gradually become less viscous to facilitate aqueous outflow after surgery.
在本研究中，开发了用于体外评估粘弹性流体（如透明质酸钠（Healon））手术性能特征的方法。透明质酸钠表现出比硫酸软骨素-6更好的手术性能。透明质酸钠的优越性能部分源于其高粘度，在剪切速率约为10 s-1时，其粘度比硫酸软骨素-6高20倍。透明质酸钠的凝胶状特性通过蠕变流动行为得到证明，其大于硫酸软骨素-6，并且对于维持前房深度很重要。结果表明，剪切粘度的阈值为80泊（在约10 s-1时）对于手术中的有效性能是必需的。还表明，每1.0 mg透明质酸钠0.42 USP单位的透明质酸酶在约2.5小时内可使剪切粘度（在约10 s-1时）降低90%。将透明质酸钠与透明质酸酶联合使用将使透明质酸钠在手术期间保持高粘度，但术后会逐渐降低粘度以促进房水流出。
