Huang E S, Miller W L
Endocrinology. 1984 Aug;115(2):513-9. doi: 10.1210/endo-115-2-513.
Treatment of ovine pituitary cell cultures with an acetone powder of porcine follicular fluid (APPFF; 50 micrograms/ml) decreased FSH secretion 60%, did not alter basal LH secretion, but increased by 2- to 3-fold the effectiveness of LHRH or D-Lys6-LHRH (10(-8) M) in releasing LH. Chromatography of APPFF on Matrex Gel Red A (MGRA) yielded a protein fraction (MGRA-IV) in which both FSH-inhibiting and LHRH-enhancing activities were enriched 8-fold. Both activities were destroyed by trypsin, but both were highly resistant to heat. The apparent mol wt of the active substance(s) was greater than 10,000. The LHRH-enhancing effect of MGRA-IV was reversible and declined, with an apparent half-life of 7 h, when MGRA-IV treatment was discontinued. There was too little estrogen in either APPFF or MGRA-IV to account for any of the activities. These results demonstrate that porcine follicular fluid contains LHRH-enhancing activity along with classical inhibin activity and that both activities may be linked in one molecule. These dual activities may be important in a number of species.
用猪卵泡液丙酮粉（APPFF；50微克/毫升）处理绵羊垂体细胞培养物，可使促卵泡素（FSH）分泌减少60%，不改变基础促黄体素（LH）分泌，但可使促性腺激素释放激素（LHRH）或D-赖氨酸6-LHRH（10⁻⁸M）释放LH的效力提高2至3倍。APPFF在基质凝胶红A（MGRA）上进行色谱分析，得到一个蛋白组分（MGRA-IV），其中FSH抑制活性和LHRH增强活性均富集了8倍。两种活性均被胰蛋白酶破坏，但对热均具有高度抗性。活性物质的表观分子量大于10,000。当停止MGRA-IV处理时，MGRA-IV的LHRH增强作用是可逆的，且以约7小时的表观半衰期下降。APPFF或MGRA-IV中的雌激素含量过少，无法解释任何一种活性。这些结果表明，猪卵泡液含有LHRH增强活性以及经典的抑制素活性，且两种活性可能存在于一个分子中。这些双重活性在许多物种中可能都很重要。
