Ohta H, Yoshikawa Y, Kai C, Yamanouchi K, Okada H
Immunology. 1984 Jul;52(3):437-44.
Horse red blood cells (HRBC) were found to be lysed when incubated with fresh normal chicken serum (NCS). By comparison of the properties of the lysis of HRBC with those of the complement-dependent lysis of sheep red blood cells (SRBC) sensitized with haemolytic antibody via the classical pathway, the following differences were observed between the two haemolytic phenomena. (i) The lysis of HRBC was independent on antibody in contrast to the antibody dependence of the lysis of sensitized SRBC. (ii) The lysis of HRBC was dependent on Mg but not on Ca ion, whereas the lysis of sensitized SRBC required both Mg and Ca ions. (iii) Treatment of NCS with carrageenan that acts as an inactivator of the first component of complement (C1) inhibited the lysis of sensitized SRBC but not the lysis of HRBC. (iv) C1 was consumed in the lysis of sensitized SRBC but not in the lysis of HRBC. (v) Cobra venom factor (CVF), C3 inactivator via the alternative complement pathway, inhibited the lysis of HRBC but not the lysis of sensitized SRBC. (vi) Minimal reaction times for the lysis of HRBC and for the lysis of sensitized SRBC were 90 and 60 min, respectively. These findings indicate that the lysis of HRBC was caused by the antibody-independent activation of complement via the alternative pathway.
发现马红细胞（HRBC）与新鲜正常鸡血清（NCS）一起孵育时会发生裂解。通过比较HRBC裂解的特性与经经典途径用溶血抗体致敏的绵羊红细胞（SRBC）的补体依赖性裂解的特性，观察到这两种溶血现象之间存在以下差异。（i）与致敏SRBC裂解的抗体依赖性相反，HRBC的裂解不依赖抗体。（ii）HRBC的裂解依赖于镁离子而非钙离子，而致敏SRBC的裂解则同时需要镁离子和钙离子。（iii）用作为补体第一成分（C1）灭活剂的角叉菜胶处理NCS可抑制致敏SRBC的裂解，但不抑制HRBC的裂解。（iv）C1在致敏SRBC的裂解中被消耗，但在HRBC的裂解中未被消耗。（v）眼镜蛇毒因子（CVF），即通过替代补体途径的C3灭活剂，可抑制HRBC的裂解，但不抑制致敏SRBC的裂解。（vi）HRBC裂解和致敏SRBC裂解的最短反应时间分别为90分钟和60分钟。这些发现表明，HRBC的裂解是由补体通过替代途径的抗体非依赖性激活引起的。
