Mizuochi T, Amano J, Kobata A
J Biochem. 1984 Apr;95(4):1209-13. doi: 10.1093/oxfordjournals.jbchem.a134711.
The susceptibility of a variety of oligosaccharides to endo-beta-N-acetylglucosaminidase D was investigated. The oligosaccharides having the structures of Man alpha 1----6 (GlcNAc beta 1----4Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(+/- Fuc alpha 1----6)GlcNAcOT, derived from complex type triantennary sugar chains, released +/- Fuc alpha 1----6GlcNAcOT upon incubation with the enzyme at almost the same rate as Man alpha 1----6(Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4GlcNAcOT. When the reaction products were reduced with NaB3H4 and analyzed by Bio-Gel P-4 column chromatography, a new radioactive peak was detected in both cases. This new radioactive oligosaccharide was confirmed to be Man alpha 1----6(GlcNAc beta 1----4Man alpha 1----3)Man beta 1----4GlcNAcOT in the former case and Man alpha 1----6(Man alpha 1----3)Man beta 1----4GlcNAcOT in the latter. These results indicated that endo-beta-N-acetylglucosaminidase D does not require the presence of a free hydroxyl group at the C-4 position of the alpha-mannosyl residue of the trisaccharide glycon: Man alpha 1----3Man beta 1----4GlcNAc beta 1----.
研究了多种低聚糖对内切-β-N-乙酰葡糖胺酶D的敏感性。源自复合型三触角糖链、具有结构Manα1----6(GlcNAcβ1----4Manα1----3)Manβ1----4GlcNAcβ1----4(+/- Fucα1----6)GlcNAcOT的低聚糖，在与该酶孵育时，释放出+/- Fucα1----6GlcNAcOT的速率几乎与Manα1----6(Manα1----3)Manβ1----4GlcNAcβ1----4GlcNAcOT相同。当用NaB3H4还原反应产物并通过Bio-Gel P-4柱色谱分析时，在两种情况下均检测到一个新的放射性峰。在前一种情况下，这种新的放射性低聚糖被确认为Manα1----6(GlcNAcβ1----4Manα1----3)Manβ1----4GlcNAcOT，在后一种情况下为Manα1----6(Manα1----3)Manβ1----4GlcNAcOT。这些结果表明，内切-β-N-乙酰葡糖胺酶D不需要三糖糖苷配基的α-甘露糖基残基的C-4位存在游离羟基：Manα1----3Manβ1----4GlcNAcβ1----。
