Weinstock S B, Kopito R R, Endemann G, Tomera J F, Marinier E, Murray D M, Brunengraber H
J Biol Chem. 1984 Jul 25;259(14):8939-44.
The shunt pathway of mevalonate metabolism (Edmond, J., and Popják, G. (1974) J. Biol. Chem. 249, 66-71) has been studied in isolated livers from fed rats perfused with physiological concentrations of variously labeled [14C]mevalonates. The measured rates of 14CO2 production were converted to rates of mitochondrial acetyl-CoA production from mevalonate by methods which take into account underestimations of metabolic rates derived from 14CO2 production. Our data confirm that the shunt pathway leads to mitochondrial acetyl-CoA. The apparent negligible rate of mevalonate shunting in liver, previously reported by others, stems from the very low contribution (congruent to 0.1%) of plasma mevalonate to total mevalonate metabolism in the liver. This contribution was assessed from the relative incorporations of 3H2O and [5-14C]mevalonate into sterols. In livers from fed rats, the shunt diverts about 5% of the production of mevalonate. The total rate of mevalonate shunting in the liver is about 200 times greater than in two kidneys. The liver is therefore the main site of mevalonate shunting in the rat.
甲羟戊酸代谢的分流途径（埃德蒙，J.，和波普雅克，G.（1974年）《生物化学杂志》249卷，66 - 71页）已在从喂食大鼠分离出的肝脏中进行研究，这些肝脏用生理浓度的各种标记的[¹⁴C]甲羟戊酸进行灌注。通过考虑因¹⁴CO₂产生而导致的代谢率低估的方法，将测得的¹⁴CO₂产生速率转换为甲羟戊酸产生线粒体乙酰辅酶A的速率。我们的数据证实分流途径会导致线粒体乙酰辅酶A的产生。其他人先前报道的肝脏中甲羟戊酸分流的表观可忽略不计的速率，源于血浆甲羟戊酸对肝脏中甲羟戊酸总代谢的贡献非常低（约为0.1%）。这种贡献是通过³H₂O和[5 - ¹⁴C]甲羟戊酸掺入甾醇的相对量来评估的。在喂食大鼠的肝脏中，分流使约5%的甲羟戊酸产生发生转向。肝脏中甲羟戊酸分流的总速率比两个肾脏中的约大200倍。因此，肝脏是大鼠中甲羟戊酸分流的主要部位。
