用于间接酶联免疫吸附测定的粗植物提取物中的病毒与戊二醛处理过的平板的结合。

Binding of viruses from crude plant extracts to glutaraldehyde-treated plates for indirect ELISA.

作者信息

Ehlers U, Paul H L

出版信息

J Virol Methods. 1984 May;8(3):217-24. doi: 10.1016/0166-0934(84)90016-8.

Abstract

A method for binding viruses from crude plant sap to ELISA microtitre plates not precoated with antibodies is described. Plates were coated with 3-(triethoxysilyl)-propylamine, and virus particles were covalently coupled to the plate surface using glutaraldehyde. Detection of trapped viruses was carried out by an indirect ELISA procedure using crude antisera or IgGs as detecting antibodies and a protein A-peroxidase conjugate. The number of virus particles attached to the plates depended on the ratio of virus concentration to plant constituents rather than on the absolute amount of virus present in the samples. Binding of virus to glutaraldehyde-treated plates was much better than to untreated plates. Possible applications of the method for studies on the relationship and identification of plant viruses are discussed.

摘要

本文描述了一种将粗制植物汁液中的病毒结合到未预先包被抗体的ELISA微量滴定板上的方法。将板用3-(三乙氧基甲硅烷基)丙胺包被，然后使用戊二醛将病毒颗粒共价偶联到板表面。使用粗抗血清或IgG作为检测抗体以及蛋白A-过氧化物酶缀合物，通过间接ELISA程序对捕获的病毒进行检测。附着在板上的病毒颗粒数量取决于病毒浓度与植物成分的比例，而不是样品中病毒的绝对量。病毒与经戊二醛处理的板的结合比与未处理的板要好得多。讨论了该方法在植物病毒关系研究和鉴定中的可能应用。