Carroll K, Lannon B, O'Kennedy R
BioResearch Ireland, Dublin.
J Immunol Methods. 1990 May 8;129(1):71-6. doi: 10.1016/0022-1759(90)90422-r.
Glutaraldehyde (GA) is commonly used to achieve the stable fixation of whole cells onto microtitre plates for use in solid-phase ELISA. Concentrations of GA greater than 0.10% (v/v) were found to result in increased levels of non-specific binding of antibodies in ELISA. However, the use of 0.05-0.025% (v/v) GA permitted the stable fixation of cells whilst minimizing the non-specific effects seen with higher GA concentrations. The binding of cells onto microtitre plates was also found to be highly variable depending on both the type of plates and the nature of the cells being used.
戊二醛（GA）通常用于将全细胞稳定固定在微量滴定板上，以用于固相酶联免疫吸附测定（ELISA）。已发现GA浓度大于0.10%（v/v）会导致ELISA中抗体非特异性结合水平升高。然而，使用0.05 - 0.025%（v/v）的GA可使细胞稳定固定，同时将较高GA浓度时出现的非特异性效应降至最低。还发现细胞在微量滴定板上的结合高度可变，这取决于板的类型和所使用细胞的性质。
