Minah G E, Chu N
Arch Oral Biol. 1984;29(6):467-71. doi: 10.1016/0003-9969(84)90028-1.
Sucrose metabolism of dental plaque which accumulated in fissure-like spaces between small bovine enamel cubes on removable U-shaped appliances was analysed using radiolabelled techniques. The appliances were worn by 8 dental students for 21 days. Half of the fissural inserts (3) on one wing of the appliance were exposed to a 10 per cent sucrose solution in normal saline and half (3) to normal saline as a control on the opposite wing, 8 x/day. At 21 days [14C]-sucrose (U) was applied directly to sucrose and saline-exposed inserts extra-orally for 60 min at 37 degrees C (non-dispersed plaques, NDP). Adjacent inserts on both sides of the appliances were removed. The plaque was dispersed in buffer by sonication, cultured and was exposed to radiolabelled sucrose for the same incubation period (dispersed plaques, DP). The following observations were made: (1) quantitative values for sucrose consumption and lactic acid, cell-bound and insoluble product, soluble polysaccharide, carbon dioxide and total volatile acid formation did not differ between sucrose and saline exposed DP or sucrose- and saline-exposed NDP; (2) all DP consumed more sucrose and formed higher quantitative levels of all products than NDP counterparts, p less than 0.001; (3) sucrose-exposed DP and sucrose exposed NDP formed proportionally more lactic acid than saline-exposed counterparts, p less than 0.05; (4) sucrose-exposed NDP formed proportionally more lactic acid and less soluble polysaccharide than sucrose exposed DP, p less than 0.025; (5) concentrations of lactobacilli were higher in sucrose exposed plaques, p less than 0.049.
采用放射性标记技术分析了可拆卸U形矫治器上小牛牙釉质小方块之间裂隙样间隙中积累的牙菌斑的蔗糖代谢情况。8名牙科学生佩戴该矫治器21天。矫治器一侧翼上的一半裂隙插入物（3个）每天8次暴露于含10%蔗糖的生理盐水中，另一侧翼上的一半（3个）作为对照暴露于生理盐水中。在第21天，将[¹⁴C] - 蔗糖（U）在37℃下经口外直接应用于暴露于蔗糖和盐水的插入物60分钟（非分散菌斑，NDP）。移除矫治器两侧相邻的插入物。通过超声处理将菌斑分散在缓冲液中，培养并在相同的孵育期内暴露于放射性标记的蔗糖中（分散菌斑，DP）。得出以下观察结果：（1）蔗糖和盐水暴露的DP或蔗糖和盐水暴露的NDP之间，蔗糖消耗、乳酸、细胞结合和不溶性产物、可溶性多糖、二氧化碳和总挥发性酸形成的定量值没有差异；（2）所有DP比NDP对应物消耗更多的蔗糖并形成更高水平的所有产物，p＜0.001；（3）蔗糖暴露的DP和蔗糖暴露的NDP比盐水暴露的对应物形成比例更高的乳酸，p＜0.05；（4）蔗糖暴露的NDP比蔗糖暴露的DP形成比例更高的乳酸和更少的可溶性多糖，p＜0.025；（5）蔗糖暴露的菌斑中乳酸杆菌浓度更高，p＜0.049。
