[使用抗天然DNA（dG-dC特异性）单克隆抗体的染色体R带分析]
[Chromosomal R-banding with an anti-native DNA (dG-dC specificity) monoclonal antibody].
作者信息
Magaud J P, Brochier J, Rimokh R, Germain D
出版信息
C R Acad Sci III. 1984;299(6):135-8.
PMID:6432233
Abstract
Chromosomal R-banding was obtained by the use of an anti ds-DNA monoclonal antibody, specific for dG-dC, at an appropriate dilution. Better results were observed when chromosome preparations were trypsinized for 10 sec. with 0.02% trypsin solution. Antibody fixation could be evidence either by immunofluorescent or by immunoenzymatic staining.
摘要
通过使用针对dG-dC的抗双链DNA单克隆抗体，并以适当的稀释度获得染色体R带。当染色体标本用0.02%胰蛋白酶溶液胰蛋白酶处理10秒时，观察到更好的结果。抗体固定可以通过免疫荧光或免疫酶染色来证实。
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