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[使用抗天然DNA(dG-dC特异性)单克隆抗体的染色体R带分析]

[Chromosomal R-banding with an anti-native DNA (dG-dC specificity) monoclonal antibody].

作者信息

Magaud J P, Brochier J, Rimokh R, Germain D

出版信息

C R Acad Sci III. 1984;299(6):135-8.

PMID:6432233
Abstract

Chromosomal R-banding was obtained by the use of an anti ds-DNA monoclonal antibody, specific for dG-dC, at an appropriate dilution. Better results were observed when chromosome preparations were trypsinized for 10 sec. with 0.02% trypsin solution. Antibody fixation could be evidence either by immunofluorescent or by immunoenzymatic staining.

摘要

通过使用针对dG-dC的抗双链DNA单克隆抗体,并以适当的稀释度获得染色体R带。当染色体标本用0.02%胰蛋白酶溶液胰蛋白酶处理10秒时,观察到更好的结果。抗体固定可以通过免疫荧光或免疫酶染色来证实。

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