Characterization of anti-N-acetyl-D-glucosamine antibodies elicited through haptenated liposomes.

Antiserum was raised in rabbits against p-aminophenyl 2-acetamido-2-deoxy-beta-D-glucoside covalently coupled to phosphatidylaminoethanol-containing lecithin liposomes. The affinity-purified antibodies were found to be IgM. The properties of the antibodies were studied by ligand inhibition of quantitative precipitation, fluorescence titration with 2-acetamido-2-deoxy-D-glucose, and complement lysis of haptenated liposomes.