Dratwa M, LeFurgey A, Tisher C C
Cell Tissue Res. 1984;236(3):585-91. doi: 10.1007/BF00217226.
Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initiated to establish whether the transformation is mediated by the cytoskeletal network and, thus, can be prevented by disruption of microtubule-microfilament function with colchicine or cytochalasin B (CB). Scanning electron microscopy revealed the characteristic branching ridges on granular cells of control bladder incubated with colchicine or CB. In contrast, transformation of ridges to discrete microvilli was observed in experimental bladders exposed to serosal hypertonicity alone or in combination with either colchicine or CB. These results suggest that the mechanism underlying hypertonicity-induced surface changes which are associated with increased water permeability does not involve either microtubules or microfilaments.
伴随浆膜高渗诱导的蟾蜍膀胱水渗透性增加，颗粒细胞的脊状表面结构会转变为单个微绒毛结构。开展本研究是为了确定这种转变是否由细胞骨架网络介导，进而能否通过用秋水仙碱或细胞松弛素B（CB）破坏微管-微丝功能来预防。扫描电子显微镜显示，用秋水仙碱或CB孵育的对照膀胱颗粒细胞上有特征性的分支脊。相比之下，在单独暴露于浆膜高渗或与秋水仙碱或CB联合暴露的实验膀胱中，观察到脊向离散微绒毛的转变。这些结果表明，与水渗透性增加相关的高渗诱导表面变化的潜在机制不涉及微管或微丝。
