Reischl E, Jelkmann W, Götz K H, Albers C, Bauer C
Comp Biochem Physiol B. 1984;78(2):443-6. doi: 10.1016/0305-0491(84)90056-7.
Oxygenation studies with the whole blood of Phrynops hilarii show a P50 of 38 torr at extracellular pH (pHe) of 7.4 which corresponds to an intracellular pH (pHi) of 7.05 at 25 degrees C. The blood CO2 Bohr effect was -0.56 when related to pHi. pHi is related to pHe by the following equation: pHi = 0.75.pHe + 1.54 (r = 0.99); pHi = 0.72. pHe + 1.72 (r = 0.96) at 10 and 25 degrees C respectively. Blood pHe, for 25 degrees C, was 7.519 +/- 0.254 (n = 6). Blood gas partial pressures were: pCO2 = 25.8 +/- 3.8 torr (n = 6); pO2 = 61.7 +/- 21.2 torr (n = 6). The major red cell phosphates, in mmole/l erythrocytes, n = 6, were: ATP (3.66 +/- 0.86); GTP (0.53 +/- 0.28); 2.3-DPG (0.32 +/- 0.12) and inorganic phosphates (2.00 +/- 0.35). The plasma inorganic ion composition, n = 6, was, in mEq/l: K+ (3.04 +/- 0.40); Na+ (148.4 +/- 12.6); Ca2+ (4.75 +/- 1.32); Cl- (106.6 +/- 5.0). Additional blood parameters of interest (n = 6) were: lactate (2.07 +/- 1.72 mM in plasma); erythrocytes/mm3 (416 X 10(3) +/- 4.6 X 10(3)); leucocytes/mm3 (44636 +/- 2618); haematocrit (%) (14.5 +/- 3.6); haemoglobin, g/dl (3.2 +/- 0.5); plasma protein g/dl (4.4 +/- 0.4); osmolarity (293 +/- 10 mOsm/l). The non-bicarbonate buffer value was -22.6 mmol/kg H2O/pH. For a constant CO2 content, delta pHe/delta t = 0.0141 +/- 0.002 (n = 18) and delta pHi/delta t = 0.0157 +/- 0.003 (n = 18).
对希氏蟾头龟全血进行的氧合研究表明，在细胞外pH值（pHe）为7.4时，P50为38托，这相当于在25摄氏度时细胞内pH值（pHi）为7.05。当与pHi相关时，血液的二氧化碳玻尔效应为-0.56。pHi与pHe的关系由以下方程表示：在10摄氏度和25摄氏度时，pHi分别等于0.75×pHe + 1.54（r = 0.99）；pHi = 0.72×pHe + 1.72（r = 0.96）。在25摄氏度时，血液pHe为7.519±0.254（n = 6）。血气分压为：pCO2 = 25.8±3.8托（n = 6）；pO2 = 61.7±21.2托（n = 6）。红细胞中主要的磷酸盐，以毫摩尔/升红细胞计，n = 6，分别为：ATP（3.66±0.86）；GTP（0.53±0.28）；2,3 - DPG（0.32±0.12）和无机磷酸盐（2.00±0.35）。血浆无机离子组成，n = 6，以毫当量/升计为：K +（3.04±0.40）；Na +（148.4±12.6）；Ca2 +（4.75±1.32）；Cl -（106.6±5.0）。其他感兴趣的血液参数（n = 6）为：乳酸（血浆中为2.07±1.72毫摩尔）；红细胞/立方毫米（416×10³±4.6×10³）；白细胞/立方毫米（44636±2618）；血细胞比容（%）（14.5±3.6）；血红蛋白，克/分升（3.2±0.5）；血浆蛋白，克/分升（4.4±0.4）；渗透压（293±10毫渗透摩尔/升）。非碳酸氢盐缓冲值为-22.6毫摩尔/千克H₂O/pH。对于恒定的二氧化碳含量，ΔpHe/Δt = 0.0141±0.002（n = 18），ΔpHi/Δt = 0.0157±0.003（n = 18）。
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