McCreery B R
Gen Comp Endocrinol. 1984 Sep;55(3):367-72. doi: 10.1016/0016-6480(84)90005-4.
Acute (5 min) graded electrical stimulation (biphasic pulses: amplitude, 50, 200, and 500 microA; frequency, 10 Hz; duration, 0.5 msec) of the medial preoptic area (including the medial and lateral septal nuclei and amygdala, pars medialis) induced significant elevations in circulating gonadotropins (more luteinizing hormone, LH, than follicle-stimulating hormone, FSH) in male bullfrogs, Rana catesbeiana. Pituitary response to all currents was rapid and of brief duration; levels of plasma gonadotropins (LH and FSH) peaked at 5 min (the end of stimulation) and decreased rapidly thereafter (LH by at least 50% after 30 min). A stimulus-response relationship was apparent; the magnitude of LH maxima was directly dependent on current intensity between 50 and 500 microA. In general, pituitary responses characteristic of acute electrical stimulation were similar to those induced by injection of mammalian gonadotropin-releasing hormone (GnRH). This is the first direct physiological demonstration of GnRH-like activity in the brain of the bullfrog.
对雄性牛蛙（牛蛙属）的内侧视前区（包括内侧和外侧隔核以及杏仁核内侧部）进行急性（5分钟）分级电刺激（双相脉冲：幅度为50、200和500微安；频率为10赫兹；持续时间为0.5毫秒），可导致循环促性腺激素（黄体生成素，LH，比促卵泡激素，FSH更多）显著升高。垂体对所有电流的反应迅速且持续时间短暂；血浆促性腺激素（LH和FSH）水平在5分钟（刺激结束时）达到峰值，此后迅速下降（30分钟后LH至少下降50%）。刺激-反应关系明显；LH最大值的幅度直接取决于50至500微安之间的电流强度。一般来说，急性电刺激的垂体反应特征与注射哺乳动物促性腺激素释放激素（GnRH）所诱导的反应相似。这是牛蛙脑中GnRH样活性的首次直接生理学证明。
