Cramer S M, Nathanael B, Horváth C
J Chromatogr. 1984 Jul 20;295(2):405-11. doi: 10.1016/s0021-9673(01)87642-1.
The assay of the complexed (ferrioxamine) and uncomplexed forms of deferoxamine, a strong iron(III) chelating agent, by reversed-phase high-performance liquid chromatography was investigated. Complex formation with trace amounts of iron in the chromatographic system hinders the assay of deferoxamine by causing appearance of unexpected peaks, reaction zones and variations in retention time. By purging the column with deferoxamine and using EDTA in the mobile phase, these adverse effects in the determination of deferoxamine were eliminated. No interference by iron contamination of the chromatographic system was found in the assay of ferrioxamine, which is facilitated by the strong absorption of the analyte at 430 nm.
研究了采用反相高效液相色谱法测定强力铁（III）螯合剂去铁胺的络合形式（铁去铁胺）和非络合形式。色谱系统中痕量铁形成的络合物会导致出现意外峰、反应区和保留时间变化，从而妨碍去铁胺的测定。通过用去铁胺吹扫色谱柱并在流动相中使用乙二胺四乙酸，消除了去铁胺测定中的这些不利影响。在铁去铁胺的测定中未发现色谱系统铁污染的干扰，这得益于分析物在430nm处有较强吸收。
