Crapper R M, Thomas W R, Schrader J W
J Immunol. 1984 Oct;133(4):2174-9.
Previously we described the persistent in vitro growth of lines of cells (persisting [P] cells) that shared many cytochemical, biochemical, and functional characteristics with mast cells and depended for their survival and growth on a specific T cell-derived factor, P cell-stimulating factor (PSF). Here we present further evidence for their identity with the T-dependent or atypical subset of mast cells and show that they retain characteristics of T-dependent mast cells when transferred in vivo. One week after the injection of P cells into the dermis of mutant Wf/Wf mice, which have a genetically determined deficiency in mast cells, large numbers of mast cells were present at the injection site, although by 2 wk or later these had disappeared. These mast cells resembled T-dependent mast cells rather than connective tissue mast cells in terms of their size and staining characteristics. Further evidence that these mast cells belonged to the T-dependent subset was that they retained their sensitivity to PSF. Thus, if P cells were injected into the dermis of Wf/Wf mice that bore in one groin a subcutaneous tumor (WEHI-3B) that produced PSF, increased numbers of mast cells were still evident at the injection site 4 wk later; this was not the case in mice bearing a non-PSF-producing variant of the same tumor. Experiments with cloned P cells generated from mice bearing the beige (bgJ/bgJ) mutation and with the giant granules of cells of this genotype used as a marker showed conclusively that the mast cells at the injection sites were derived from the injected P cells. P cells sensitized in vitro with monoclonal antigen-specific IgE or IgG1 antibodies and then injected intracutaneously into W/Wv mice transferred local cutaneous anaphylactic responses. P cells sensitized with IgG1 transferred local cutaneous anaphylactic responses to rats. These results support the view that P cell lines are cognate with the atypical or T-dependent subset of mast cells and that these cells retain their functional capabilities when injected in vivo.
此前我们描述了细胞系（持续存在的[P]细胞）在体外的持续生长，这些细胞与肥大细胞具有许多细胞化学、生化和功能特征，并且其存活和生长依赖于一种特定的T细胞衍生因子，即P细胞刺激因子（PSF）。在此我们提供了进一步的证据，证明它们与T细胞依赖性或非典型肥大细胞亚群相同，并表明它们在体内转移时保留了T细胞依赖性肥大细胞的特征。将P细胞注射到基因决定肥大细胞缺陷的突变型Wf/Wf小鼠的真皮中一周后，注射部位出现了大量肥大细胞，尽管到2周或更晚时这些肥大细胞已经消失。这些肥大细胞在大小和染色特征方面类似于T细胞依赖性肥大细胞，而非结缔组织肥大细胞。这些肥大细胞属于T细胞依赖性亚群的进一步证据是它们对PSF仍保持敏感性。因此，如果将P细胞注射到腹股沟处有皮下肿瘤（WEHI-3B）能产生PSF的Wf/Wf小鼠的真皮中，四周后注射部位仍明显可见肥大细胞数量增加；而在携带同一肿瘤的不产生PSF变体的小鼠中则并非如此。用携带米色（bgJ/bgJ）突变的小鼠产生的克隆P细胞以及将该基因型细胞的巨大颗粒用作标记进行的实验最终表明，注射部位的肥大细胞源自注射的P细胞。用单克隆抗原特异性IgE或IgG1抗体在体外致敏的P细胞，然后皮内注射到W/Wv小鼠体内，可转移局部皮肤过敏反应。用IgG1致敏的P细胞可将局部皮肤过敏反应转移给大鼠。这些结果支持这样一种观点，即P细胞系与非典型或T细胞依赖性肥大细胞亚群相关，并且这些细胞在体内注射时保留了其功能能力。
