Toukdarian A E, Lidstrom M E
J Gen Microbiol. 1984 Jul;130(7):1827-37. doi: 10.1099/00221287-130-7-1827.
A new obligate methanotroph was isolated and characterized. It was classified as a 'Methylosinus' species and named 'Methylosinus' sp. strain 6. Nitrogen metabolism in 'Methylosinus' 6 was found to be similar to other Type II methanotrophs, including the assimilation of nitrogen exclusively by the glutamine synthetase/glutamate synthase system. However, unlike other Type II methanotrophs, it appeared that glutamine synthetase activity was regulated by adenylylation in this organism. 'Methylosinus' 6 was grown in continuous culture with either dinitrogen or nitrate as sole nitrogen source under various dissolved oxygen tensions. Higher rates of methane oxidation and a more developed intracytoplasmic membrane system were found at lower oxygen tensions with nitrate as the nitrogen source but at higher oxygen tensions with dinitrogen as the nitrogen source. This suggested that carbon metabolism was influenced by nitrogen metabolism in this organism.
分离并鉴定了一种新的专性甲烷氧化菌。它被归类为“甲基弯曲菌”属，并命名为“甲基弯曲菌”sp.菌株6。发现“甲基弯曲菌”6中的氮代谢与其他II型甲烷氧化菌相似，包括仅通过谷氨酰胺合成酶/谷氨酸合酶系统同化氮。然而，与其他II型甲烷氧化菌不同的是，在这种生物体中，谷氨酰胺合成酶活性似乎受腺苷酸化调节。“甲基弯曲菌”6在不同溶解氧张力下，以氮气或硝酸盐作为唯一氮源进行连续培养。当以硝酸盐作为氮源时，在较低氧张力下发现较高的甲烷氧化速率和更发达的胞内膜系统；而以氮气作为氮源时，在较高氧张力下出现这种情况。这表明该生物体中的碳代谢受氮代谢影响。
