Morris C J, Wainwright A C, Steven M M, Blake D R
Virchows Arch A Pathol Anat Histopathol. 1984;404(1):75-85. doi: 10.1007/BF00704252.
Using a computerized electron-probe X-ray microanalytical technique to measure phosphorus/iron ratios we have defined the iron saturation of ferritin in vitro from prepared ferritin standards of known iron loading. This technique has been applied to the study of 5 haemophilic synovial membranes. At light microscope level the distribution and relationship of iron/ferritin were defined using Perls' reaction and an immunoperoxidase technique respectively. The synovia from all cases contained intra and extra-cellular deposits of Perls' positive material which were granular in nature in the most superficial synovial cells. There were increasing numbers of pheomorphic (1-12 micron diameter ovate bodies in the deeper synovial layers. Immunoperoxidase ferritin staining produced a strongly positive reaction in the granular material but the ovate bodies were negative with the exception of some peripheral staining. X-ray microanalysis showed the granular material to be highly iron saturated ferritin and the ovate bodies to be almost pure iron. We suggest that iron saturated ferritin in the synovial membrane could increase/perpetuate inflammation by promoting lipid peroxidation.
我们使用计算机化电子探针X射线微量分析技术来测量磷/铁比率，通过已知铁负载量的制备铁蛋白标准品在体外确定了铁蛋白的铁饱和度。该技术已应用于5个血友病滑膜的研究。在光学显微镜水平，分别使用Perls反应和免疫过氧化物酶技术确定铁/铁蛋白的分布和关系。所有病例的滑膜均含有细胞内和细胞外Perls阳性物质沉积物，在最表层滑膜细胞中为颗粒状。在较深的滑膜层中，多形性（直径1-12微米的卵形小体）数量不断增加。免疫过氧化物酶铁蛋白染色在颗粒物质中产生强阳性反应，但卵形小体除一些周边染色外呈阴性。X射线微量分析表明，颗粒物质是高铁饱和铁蛋白，卵形小体几乎是纯铁。我们认为滑膜中的铁饱和铁蛋白可能通过促进脂质过氧化来加重/使炎症持续。
