Beretta A, Gullberg M, Larsson E L, Möller G
Ann Immunol (Paris). 1984 May-Jun;135C(3):365-73. doi: 10.1016/s0769-2625(84)80966-6.
The process of activation of T lymphocytes by ionophoric substances was analysed according to current concepts of T-lymphocyte activation. Proliferation induced by the ionophore A23-187 was found to be strictly dependent on the exogenous availability of growth factors. Lack of production of growth factors in spleen cell cultures containing the ionophore A23-187 explained the failure to directly stimulate mouse T-lymphocyte proliferation. It was concluded that the main action of A23-187 is the induction of expression of growth receptors, and that the increase in Ca++ uptake is, in itself, not sufficient to induce mitotic events.
根据目前关于T淋巴细胞激活的概念，分析了离子载体物质对T淋巴细胞的激活过程。发现离子载体A23 - 187诱导的增殖严格依赖于生长因子的外源性供应。在含有离子载体A23 - 187的脾细胞培养物中缺乏生长因子的产生，解释了未能直接刺激小鼠T淋巴细胞增殖的原因。得出的结论是，A23 - 187的主要作用是诱导生长受体的表达，并且Ca++摄取的增加本身不足以诱导有丝分裂事件。
